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Mass Spectrometry Core (BIDMC)

Location: Mass Spectrometry Core, Beth Israel Deaconess Medical Center, CLS-425, Boston, MA 02115

Summary:

The mass spectrometry, proteomics, metabolomics and lipidomics facility at Beth Israel Deaconess Medical Center was established in 2004 led by Dr. John Asara and continues to grow rapidly. We provide excellent proteomics and metabolomics services with low prices and fast turnaround times. We have cutting edge instruments operated at optimal sensitivity and work and publish with world class researchers. We also maintain an active internal cancer proteomics and metabolomics research program with collaborative efforts. Our expertise is in identifying and quantifying protein modifications and dynamic protein-protein interactions in addition to polar metabolomics profiling, flux analysis and lipidomics profiling. We accept collaborations from anywhere in the world and look forward to helping you and progressing your research to the next level.

Affiliations:

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Instruments

  • AB SCIEX QTRAP 5500 mass spectrometer ( Liquid chromatography mass spectrometry platform )

    Instrument used for metabolite identification, detection and confirmation of low-level pesticides, and protein/peptide quantitation. "The AB SCIEX QTRAP® 5500 LC/MS/MS system is the next-generation technology which is designed to excel at metabolite identification, detection and confirmation of low-level pesticides, and protein/peptide quantitation for biomarker verification and validation. Redesigned from the ground up using our new, fast eQTM electronics, but bred from our industry standard triple quadrupole line of mass spectrometers, the QTRAP® 5500 system brings in a new era of performance.

    The QTRAP® 5500 system also houses the most sensitive ion trap in the world-the all new, patented Linear AcceleratorTM trap along with offering ultra-fast scan speeds, and full MS3 capabilities."

  • AB SCIEX QTRAP 5500 mass spectrometer ( Liquid chromatography mass spectrometry platform )

    Used for metabolite identification, detection and confirmation of low-level pesticides and protein/peptide quantitation. "The AB SCIEX QTRAP® 5500 LC/MS/MS system is the next-generation technology which is designed to excel at metabolite identification, detection and confirmation of low-level pesticides, and protein/peptide quantitation for biomarker verification and validation. Redesigned from the ground up using our new, fast eQTM electronics, but bred from our industry standard triple quadrupole line of mass spectrometers, the QTRAP® 5500 system brings in a new era of performance.

    The QTRAP® 5500 system also houses the most sensitive ion trap in the world-the all new, patented Linear AcceleratorTM trap along with offering ultra-fast scan speeds, and full MS3 capabilities."

  • Michrom Bioresources Paradigm 2D-HPLC ( Liquid chromatography instrument )

    Used for protein factionation.

  • Proxeon EASY-nLC ( Liquid chromatography instrument )

    Two copies of this instrument available. Used for Liquid Chromatography. Nanoliter flow rates, very reproducible and robust.

  • Shimadzu Prominence UFLC ( High performance liquid chromatography instrument )

    Used for Liquid Chromatography. Nanoliter flow rates, very reproducible and robust.

  • Thermo Proxeon nLC II ( Liquid chromatography mass spectrometry platform )

    Nanoliter flow rates, very reproducible and robust.

  • Thermo Scientific Elite Orbitrap ( Ion trap mass spectrometer )

    The Elite represents the ultimate proteomics instrument for speed, sensitivity, resolution and throughput.

  • Thermo Scientific LTQ Orbitrap XL-ETD ( Mass spectrometer )

    Provides proteomics solution for protein ID, characterization and quantitation. Ultra high resolution and mass accuracy, HCD collision cell, ETD fragmentation for labile modifications.

  • Thermo Scientific Q Exactive Plus ( Mass spectrometer )

    QExactive Plus Orbitrap high resolution mass spectrometer for a future lipidomics and untargeted metabolomics platform. (This is considered one of Thermo's fastest and most sensitive instruments.)

Protocols

  • Metabolomics profiling protocol ( Protocol )

    Protocol uses MS/MS or LC/MS to analyze the various metabolites (small molecules) in the serum, plasma or in cells that have been organically extracted to remove DNA, RNA, proteins, membranes etc.

  • Protein acetylation protocol ( Protocol )

    "Protein lysine acetylation, referring to acetylation of the ɛ-amino group of a lysine residue, [is] an important post-translational modification for regulating protein functions in various organisms. Lysine acetylation is a rapidly reversible and precisely controlled covalent modification that serves as a simple on/off switch or participates in a codified manner with other post-translational modifications to regulate protein functions in different cellular and developmental processes. This unit describes and discusses methods used for in vitro and in vivo determination of lysine acetylation."
    Curr. Protoc. Protein Sci. 54:14.11.1-14.11.17.

  • Protein complex identification protocol ( Protocol )

    The protocol describes how to identify all the protein species (usually by identifying unique peptides in the mixture) in a protein complex.

  • Protein complex stoichiometry protocol ( Protocol )

    The protocol describes how to determine the number of protein molecules in a complex.

  • Protein phosphorylation protocol ( Protocol )

    "Protein phosphorylation is a post-translational modification of proteins in which a serine, a threonine or a tyrosine residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Regulation of proteins by phosphorylation is one of the most common modes of regulation of protein function, and is often termed "phosphoregulation". In almost all cases of phosphoregulation, the protein switches between a phosphorylated and an unphosphorylated form, and one of these two is an active form, while the other one is inactive."
    http://en.wikipedia.org/wiki/Protein_phosphorylation

  • Protein ubiquitination protocol ( Protocol )

  • Protein-protein interactions protocol ( Protocol )

    The protocol describes how to monitor whether or not two (or more) proteins of interest interact with each other.

  • Quantification of phosphorylation sites protocol ( Protocol )

    "Protein phosphorylation is a post-translational modification of proteins in which a serine, a threonine or a tyrosine residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Regulation of proteins by phosphorylation is one of the most common modes of regulation of protein function, and is often termed "phosphoregulation". In almost all cases of phosphoregulation, the protein switches between a phosphorylated and an unphosphorylated form, and one of these two is an active form, while the other one is inactive."
    http://en.wikipedia.org/wiki/Protein_phosphorylation

Services


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Last updated: 2015-01-15T13:18:46.463-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016