eagle-i Harvard UniversityHarvard University
See it in Search

Cellular Imaging Core (BCH)

Location: Children's Hospital, Division of Neuroscience, 300 Longwood Avenue, CLS13035, Boston, MA 02115

Summary:

The Imaging Core provides access to standard digital and laser scanning confocal microscopy, multiphoton microscopy, unbiased stereology, and automated confocal microscopy for large scale genome-wide screens. In addition, the Core has substantial capabilities in quantitative and three-dimensional volumetric image reconstruction.

Affiliations:

People:

Resources:

Instruments

Services

Software

  • ImageJ ( Software )

    Available on two workstations; free software package specifically tailored for fluorescence microscopy. "ImageJ is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.4 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux.

    It can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. It can read many image formats including TIFF, GIF, JPEG, BMP, DICOM, FITS and "raw". It supports "stacks", a series of images that share a single window. It is multithreaded, so time-consuming operations such as image file reading can be performed in parallel with other operations.

    It can calculate area and pixel value statistics of user-defined selections. It can measure distances and angles. It can create density histograms and line profile plots. It supports standard image processing functions such as contrast manipulation, sharpening, smoothing, edge detection and median filtering."

  • LSM 510 ( Software )

    Available for workstation 1. Off-line version of LSM 510 confocal microscope.

  • MetaMorph ( Software )

    Available for workstation 2. "MetaMorph® Microscopy Automation & Image Analysis Software is the industry standard for automated microscope acquisition, device control, and image analysis, bringing microscopists greater understanding of cell morphology, function, and behavior."

  • Zeiss LSM Image Browser ( Software )

    Available for workstation 2. Image processing software.


Web Links:

Last updated: 2017-10-25T08:46:45.928-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016