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ICCB-Longwood Screening Facility (HMS)

Director: Shamu, Caroline, Ph.D.

Location: Harvard Medical School, 250 Longwood Avenue, Seeley G. Mudd Building Rooms: 604, 605, 606, 607, 609, 611, 612; Building C Room C-607, Boston, MA, 02115

Summary:

The ICCB-Longwood Screening Facility provides resources and assistance for designing, conducting, and interpreting high-throughput chemical and siRNA screens. ICCB-Longwood is staffed by full-time personnel with expertise in lab automation, biochemistry, cellular and molecular biology, microbiology, HTS microscopy, and data analysis. The facility employs a staff-assisted screening model, in which investigators using the facility are provided with access to compound and siRNA libraries, and training in the use of some instruments, such as liquid handling equipment, plate readers, and screening microscopes. Staff members provide assistance at all stages of the screening process and perform complex automation steps.

Affiliations:

People:

Resources:

Instruments

Reagents

  • ActiMol TimTec 1 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased jointly by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) and the Broad Institute-Chemical Biology Platform in May 2005. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • Ambion Human 1: Druggable Genome ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Silencer Select Neg.Control #1 siRNA (Ambion Cat#: 439084x); Kinesin family member 11 (KIF11) (Ambion Cat#: AM4639).

    This library is the Ambion Silencer Select siRNA druggable genome pools. The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    ICCBL screeners interested in screening this library should contact Caroline Shamu for more details.

  • Ambion Human 1: Mimics ( miRNA library )

    384-well plate format.
    Concentration Information: 1uM in buffer.
    Control Information: Pre-miR miRNA Precursor Molecules-Neg.Control #1 (Ambion Cat#: AM17110); Human PLK1 siRNA SMARTPool (Dharmacon Cat#: M-003290-01); Pre-miR miRNA Precursor Molecules-Neg. Control #2 (Ambion Cat#: AM17111).

    This library is the Ambion Human Pre-miR miRNA Mimic Library and was purchased in the Summer of 2009. The design of this library was based on miRBase release 13.0. The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Ambion Human 2: Remaining Genome ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Silencer Select Neg.Control #1 siRNA (Ambion Cat#: 439084x); Kinesin family member 11 (KIF11) (Ambion Cat#: AM4639).

    This library is the Ambion Silencer Select siRNA remaining genome pools. The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    ICCBL screeners interested in screening this library should contact Caroline Shamu for more details.

  • Asinex 1 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO

    This collection was purchased by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) in February 2006. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • Biomol 4 - FDA Approved Library ( Known bioactives collection chemical library )

    384-well Plate format.
    Concentration Information: 2mg/mL in DMSO.

    This collection was plated in October 2009 and was donated to ICCBL/NSRB by Steve Elledge (Harvard Medical School).

    According to BIOMOL all of the compounds in this collection "have known and well-characterized bioactivites and have undergone safety and bioavailability testing..." They were "carefully selected to maximize chemical and pharmacological diversity" in this category of compounds.

    More information about this collection can be found on the Biomol website.

  • Biomol ICCB-L Known Bioactives 2012 ( Known bioactives collection chemical library )

    384-well plate format.
    Concentration information: Variable from 5 mg/ml (~13 mM) down to 0.1 mM based upon potency and availability. Please refer to Screensaver for specific compound concentration.

    This library is available from Enzo Life Sciences and jointly purchased by an NIH Center grant to Dr. Alan D’Andrea (DFCI) and the Assay Development and Screening Facility (Children’s Hospital Boston) in December 2012 and plated in January 2013.

    It is based on the ICCB-Longwood Bioactives 1 collection (PL-0684-0685). This collection is nearly identical to the compounds that were in the high concentration of plates 1361-1362, plates 1791-1792 and 1989-1990 (4 compound difference). It was assembled by BIOMOL, working together with ICCB-Longwood, to provide a library of compounds that affect a wide variety of biological pathways relevant to ICCB-Longwood screening projects. The collection includes many classes of compounds including ion channel blockers, GPCR ligands, second messenger modulators, nuclear receptor ligands, actin and tubulin ligands, kinase inhibitors, protease inhibitors, gene regulation agents, lipid biosynthesis inhibitors, as well as other well-characterized compounds that perturb cell pathways. We hope this collection will help both in assay development and in target identification. Because the compounds are plated at a high concentration, we suggest these 2 plates are not the first ones screened when an assay is initiated at ICCB-L.

  • Bionet 1 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    The Bionet collection was purchased jointly with the Lab for Drug Discovery for Neurodegeneration (LDDN) in January 2002. It was part of a total purchase of more than 50,000 new commercial compounds. These compounds are similar in complexity to the ChemBridge DiverSet E. However, this collection was compiled so that the molecules have an increased probability of favorable physical-chemical properties and a lack of functional groups that might cause toxicity or reduce stability. Resupply cost is $20 - $35 per mg per compound via Ryan Scientific.

  • Bionet 2 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased jointly with the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE) and the Laboratory for Drug Discovery for Neurodegeneration (LDDN) in June 2004 . Funding was provided by NERCE and the Dana Farber/Harvard Cancer Center (DF/HCC) Cancer Drug Assays program (CDA). With the assistance of Dr. Tudor Oprea at the University of New Mexico, these compounds were specifically chosen to increase the overall diversity of the ICCB collection, and were also selected for favorable physico-chemical properties, such as solubility, decreased toxicity, and increased stability.

  • CEREP ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    The CEREP collection was jointly purchased with the Lab for Drug Discovery for Neurodegeneration (LDDN) in December 2001. It was part of a total purchase of more than 50,000 new commercial compounds. These compounds are similar in complexity to the ChemBridge DiverSet E. However, this collection was compiled so that the molecules have an increased probability of favorable physical-chemical properties and a lack of functional groups that might cause toxicity or reduce stability.

    CEREP does not keep stocks of compounds. Re-orders are custom synthesized for each request and a minimum of 2 mgs of each compound must be ordered. Re-supply cost is $260 -$320/2 mg. Re-supply cost is $260 -$320/2 mg. Compounds can be ordered directly from CEREP.

  • ChemBridge 3 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) in February 2006. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • ChemDiv 1 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This ChemDiv collection was jointly purchased with the Lab for Drug Discovery for Neurodegeneration (LDDN) in December 2001, based on the ChemDiv collections that were available at that time. It was part of a total purchase of more than 50,000 new commercial compounds. This collection was compiled so that the molecules have an increased probability of favorable physical-chemical properties and a lack of functional groups that might cause toxicity or reduce stability.

  • ChemDiv 2 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased jointly with the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE) and the Laboratory for Drug Discovery for Neurodegeneration (LDDN) in June 2004 . Funding was provided by NERCE and the Dana Farber/Harvard Cancer Center (DF/HCC) Cancer Drug Assays program (CDA). With the assistance of Dr. Tudor Oprea at the University of New Mexico, these compounds were specifically chosen to increase the overall diversity of the ICCB-Longwood collection, and were also selected for favorable physico-chemical properties, such as solubility, decreased toxicity, and increased stability.

  • ChemDiv 3 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased jointly by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) and the Broad Institute-Chemical Biology Platform in May 2005. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • ChemDiv 4 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) in February 2006. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • ChemDiv 6 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 10mM in DMSO.

    The ChemDiv 6 library is a select set of 44,000 public domain compounds. This fully annotated, high quality, collection was selected by drug discovery experts to provide drug-like qualities, chemical diversity, and potential for structure activity relationship follow-up experiments.

    ICCBL screeners interested in screening this library should contact Caroline Shamu for more details.

  • ChemDiv Antimitotic Collection ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection is called "antimitotic" for historical reasons and, in fact, does not consist only of compounds with verified antimitotic activity. It was selected by a few ICCB screeners as a subset of the ChemDiv1 library. If you have questions about the library, please contact the ICCB-Longwood Director.

  • Dharmacon Human 1: Kinases-Phosphatases ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Human PLK1 SMARTpool Dharmacon Cat#: M-003290-01; siControl non-targeting siRNA #2 Dharm. Cat#: D-001206-14; siGLO RISC-free siRNA.

    This collection of siGENOME siRNAs was purchased in March 2006 as part of the RNAi Global Consortium. The design of this library is based on RefSeq versions 5 through 8. The siRNAs are arrayed as SMARTpools.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Dharmacon Human 2: GPCRs ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Human PLK1 SMARTpool Dharmacon Cat#: M-003290-01; siControl non-targeting siRNA #2 Dharm. Cat#: D-001206-14; siGLO RISC-free siRNA.

    This collection of siGENOME siRNAs was purchased in March 2006 as part of the RNAi Global Consortium. The design of this library is based on RefSeq versions 5 through 8. The siRNAs are arrayed as SMARTpools.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Dharmacon Human 3: Remaining Druggable Genome ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Human PLK1 SMARTpool Dharmacon Cat#: M-003290-01; siControl non-targeting siRNA #2 Dharm. Cat#: D-001206-14; siGLO RISC-free siRNA.

    This collection of siGENOME siRNAs was purchased in March 2006 as part of the RNAi Global Consortium. The design of this library is based on RefSeq versions 5 through 8. The siRNAs are arrayed as SMARTpools.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Dharmacon Human 4: Remaining Genome ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Human PLK1 SMARTpool Dharmacon Cat#: M-003290-01; siControl non-targeting siRNA #2 Dharm. Cat#: D-001206-14; siGLO RISC-free siRNA.

    This collection of siGENOME siRNAs was purchased in March 2006 as part of the RNAi Global Consortium. The design of this library is based on RefSeq versions 5 through 8. The siRNAs are arrayed as SMARTpools.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Dharmacon Human 5: RefSeq27 ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Human PLK1 SMARTpool Dharmacon Cat#: M-003290-01; siControl non-targeting siRNA #2 Dharm. Cat#: D-001206-14.

    This collection of siRNAs was purchased in February 2010. Dharmacon created this new collection as an update ("reversion") of their 2006 Dharmacon siGENOME library. It contains siRNA SMARTpools targeting a subset of the genes present in the Dharmacon siGENOME collection (ICCB Dharmacon Human 1-4). The design of this library is based on RefSeq version 27; Dharmacon Human 1-4 are based on RefSeq version 5 through 8. The siRNAs are arrayed as SMARTpools.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Dharmacon Human DUB 1 ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Mouse PLK1 SMARTpool Dharmacon Cat#: M-040566-00 ***Note: an error was made when plating the Human DUBs1 library and Mouse PLK1 was plated instead of Human PLK1, therefore if you would like Human PLK1 to be run with your assay please plate some yourself; siControl non-targeting siRNA #2.

    This collection of Human Deubiquitinating Enzymes was purchased in December 2006, Dharmacon Catalog #: G-004700. The siRNAs are arrayed as SMARTpools. All of the genes in this collection are also represented in the ICCBL Dharmacon Whole Human libraries (likely in plates 50,006-50,090).

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    ICCBL screeners interested in screening this library should contact Caroline Shamu for more details.

  • Dharmacon Mouse 1: Kinases-Phosphatases ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Mouse PLK1 SMARTpool Dharmacon Cat#: M-040566-00; siControl non-targeting siRNA #2; siGLO RISC-free siRNA.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Dharmacon Mouse 2: GPCRs ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Mouse PLK1 SMARTpool Dharmacon Cat#: M-040566-00; siControl non-targeting siRNA #2; siGLO RISC-free siRNA.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Dharmacon Mouse 3: Remaining Druggable Genome ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Mouse PLK1 SMARTpool Dharmacon Cat#: M-040566-00; siControl non-targeting siRNA #2; siGLO RISC-free siRNA.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Dharmacon Mouse 4: Remaining Genome ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Mouse PLK1 SMARTpool Dharmacon Cat#: M-040566-00; siControl non-targeting siRNA #2; siGLO RISC-free siRNA.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • EMD Chemicals kinase inhibitor 1 ( Known bioactives collection chemical library )

    384-well plate format.
    Concentration Information: 10mM in DMSO.

    This is a collection of kinase inhibitors purchased from EMD Biosciences in August 2010 and provided to ICCBL by Dr. Nathanael Gray. This collections includes three libraries sold by EMD as, InhibitorSelect 96-Well Protein Kinase Inhibitor I (cat #: 539744, 80 compounds), InhibitorSelect 96-Well Protein Kinase Inhibitor II (cat #: 539745, 80 compounds) and InhibitorSelect 96-Well Protein Kinase Inhibitor III (cat #: 539746, 84 compounds).

    NOTE: Permission is required to screen these library plates!

  • Enamine 1 ( Synthetic chemical library )

    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased jointly with the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE) and the Laboratory for Drug Discovery for Neurodegeneration (LDDN) in June 2004 . Funding was provided by NERCE and the Dana Farber/Harvard Cancer Center (DF/HCC) Cancer Drug Assays program (CDA). With the assistance of Dr. Tudor Oprea at the University of New Mexico, these compounds were specifically chosen to increase the overall diversity of the ICCB-Longwood collection, and were also selected for favorable physico-chemical properties, such as solubility, decreased toxicity, and increased stability.

  • Enamine 2 ( Synthetic chemical library )

    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased by a NIH U01 grant to Dr. Junying Yuan, HMS Cell Biology Department, in September 2006. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • Enamine 2a ( Synthetic chemical library )

    Concentration Information: 5 mg/ml in DMSO.

    This plate is a continuation of the library Enamine 2 and contains the same compounds as plate 1715 from that library, however in a different order. If you have already screened plate 1715 you do not need to screen this plate.

    The Enamine 2 collection was purchased by a NIH U01 grant to Dr. Junying Yuan, HMS Cell Biology Department, in September 2006. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • Exiqon Human 1: Inhibitors ( miRNA library )

    384-well plate format.
    Concentration Information: 1uM in buffer.
    Control Information: Scramble-miR; Control miRCURY knockdown probe (Exiqon Cat#:199002-00); Human PLK1 siRNA SMARTPool (Dharmacon Cat#: M-003290-01); Sense miR-159; Control miRCURY knockdown probe (Exiqon Cat#: 199003-00).

    This library is the Exiqon Human miRCURY miRNA Inhibitor Library and was acquired in the Summer of 2009. The design of this library was based on miRBase release 13.0.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Gray Kinase Inhibitor Focused Library ( Chemical library )

    384-well plate format.
    Concentration Information: 10mM in DMSO.

    This is a kinase inhibitor-focused library provided by Dr. Nathanael Gray (Dana Farber Cancer Institute). It is comprised of validated and suspected ATP-site kinase inhibitors that target active and inactive kinase conformations. NOTE: Permission is required to screen these library plates!

  • I.F. Lab 2 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased jointly with the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE) and the Laboratory for Drug Discovery for Neurodegeneration (LDDN) in June 2004 . Funding was provided by NERCE and the Dana Farber/Harvard Cancer Center (DF/HCC) Cancer Drug Assays program (CDA). With the assistance of Dr. Tudor Oprea at the University of New Mexico, these compounds were specifically chosen to increase the overall diversity of the ICCB-Longwood collection, and were also selected for favorable physico-chemical properties, such as solubility, decreased toxicity, and increased stability.

  • ICBG 10 - Sherman Lab Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: 15mg/mL.

    This library was plated and added to the collection in November 2008. Production of this library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy (Harvard Medical School) and to Dr. David Sherman (University of Michigan). The library contains partially purified extracts. Crude extracts from marine bacteria, actinomycetes and cyanobacteria were fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy. Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 11 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in Summer 2008. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 12 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in Fall 2008. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 13 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in March 2009. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 14 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in March 2009. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 15 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in June 2009. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 16 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in July 2009. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 17 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in October 2009. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 2 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in April 2005. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of 'hit' compounds from extract samples.

  • ICBG 4 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in December 2006. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 5 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in Summer 2007. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 6 - Sherman Lab Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: 15mg/mL.

    This library was plated and added to the collection in March 2007. Production of this library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy (Harvard Medical School) and to Dr. David Sherman (University of Michigan). The library contains partially purified extracts. Crude extracts from marine bacteria, actinomycetes and cyanobacteria were fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy. Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 7 - Sherman Lab Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: 15mg/mL.

    This library was plated and added to the collection in February 2008. Production of this library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy (Harvard Medical School) and to Dr. David Sherman (University of Michigan). The library contains partially purified extracts. Crude extracts from marine bacteria, actinomycetes and cyanobacteria were fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy. Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 8 - Sherman Lab Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: 15mg/mL.

    This library was plated and added to the collection in February 2008. Production of this library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy (Harvard Medical School) and to Dr. David Sherman (University of Michigan). The library contains partially purified extracts. Crude extracts from marine bacteria, actinomycetes and cyanobacteria were fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy. Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICBG 9 - Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in Summer 2008. Production of this fungal extract library was funded by an International Cooperative Biodiversity Groups (ICBG) grant from the NIH to Dr. Jon Clardy. The library contains partially purified extracts from diverse organisms that grow in Costa Rica, including lichens, cyanobacteria, sponges, and endophytic fungi (fungi that live in higher plants), and that have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • ICCBL Pilot 1 ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Human PLK1 SMARTpool Dharmacon Cat#: M-003290-01; siControl non-targeting siRNA's #2-#5.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    This library should be used to pilot siRNA screening assays. It replaces the Mitchison Kinase library previously used for piloting purposes. The ICCB-L Pilot I library is a custom library of SMARTpools purchased from Dharmacon Thermo Fisher in July 2010. The majority of the library comprises siRNA pools directed against kinases that were selected based on hit frequency from previous siRNA screens at the ICCB-L. All of the 237 siRNA pools in this collection were identified as hits in at least two different ICCB-L siRNA screens. To download an Excel spreadsheet list of the SMARTpools in this library, please click here.

  • Invitrogen Human 1: Stealth Kinases-Phosphatases ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    ICCB-Longwood screeners interested in screening this library should contact Caroline Shamu or Jennifer Smith for more details.

  • Invitrogen Human 2: Stealth Remaining Druggable ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    ICCB-Longwood screeners interested in screening this library should contact Caroline Shamu or Jennifer Smith for more details.

  • Invitrogen Human 3: QC Supplement ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    ICCB-Longwood screeners interested in screening this library should contact Caroline Shamu or Jennifer Smith for more details.

  • Life Chemicals 1 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) in February 2006. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • Maybridge 3 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased jointly with the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE) and the Laboratory for Drug Discovery for Neurodegeneration (LDDN) in June 2004 . Funding was provided by NERCE and the Dana Farber/Harvard Cancer Center (DF/HCC) Cancer Drug Assays program (CDA). With the assistance of Dr. Tudor Oprea at the University of New Mexico, these compounds were specifically chosen to increase the overall diversity of the ICCB-Longwood collection, and were also selected for favorable physico-chemical properties, such as solubility, decreased toxicity, and increased stability.

  • Maybridge 4 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased jointly by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) and the Broad Institute-Chemical Biology Platform in May 2005. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • Maybridge 5 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This collection was purchased by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) in February 2006. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • Maybridge mixed commercial plate 5 ( Synthetic chemical library )

    384-well plate format.
    Concentration Information: 5 mg/ml in DMSO.

    This plate contains compounds from ChemDiv and Maybridge. This collection was purchased jointly by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) and the Broad Institute-Chemical Biology Platform in May 2005. These compounds were specifically chosen by Dr. Tudor Oprea's group at the University of New Mexico to increase the overall diversity of the ICCB-Longwood collection, as well as for favorable physico-chemical properties such as solubility, decreased toxicity, and increased stability.

  • Microsource 1 - US Drug Collection ( Known bioactives collection chemical library )

    384-well plate format.
    Concentration Information: 2mg/mL in DMSO.

    This collection was plated in October 2009 and was donated to ICCBL/NSRB by Steve Elledge (Harvard Medical School).

    According to Microsource, the compounds in this collection "have reached clinical trial stages in the USA. Each compound has been assigned USAN or USP status and is included in the USP Dictionary (U.S. Pharmacopeia, 2005), the authorized list of established names for drugs in the USA". A list of compounds and the Excel or SD file with this information can be downloaded at the Microsource site.

  • Mitchison Kinase 1 ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.
    Control Information: Human PLK1 SMARTpool Dharmacon Cat#: M-003290-01; siControl non-targeting Pool; siGLO RISC-free siRNA.

    Dharmacon SMARTpools are a collection of RNAi Kinases, generously provided by the laboratory of Tim Mitchison. Plated June 2006.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • MMV 1 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the ICCB-L collection in November 2007. Production of this fungal extract library was funded by a Medicines for Malaria Venture (MMV) grant to Dr. Jon Clardy. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • MMV 2 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the ICCB-L collection in November 2008. Production of this fungal extract library was funded by a Medicines for Malaria Venture (MMV) grant to Dr. Jon Clardy. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • MMV 3 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the ICCB-L collection in January 2009. Production of this fungal extract library was funded by a Medicines for Malaria Venture (MMV) grant to Dr. Jon Clardy. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • MMV 4 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the ICCB-L collection in March 2009. Production of this fungal extract library was funded by a Medicines for Malaria Venture (MMV) grant to Dr. Jon Clardy. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • MMV 5 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the ICCB-L collection in March 2009. Production of this fungal extract library was funded by a Medicines for Malaria Venture (MMV) grant to Dr. Jon Clardy. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • MMV 6 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the ICCB-L collection in June 2009. Production of this fungal extract library was funded by a Medicines for Malaria Venture (MMV) grant to Dr. Jon Clardy. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • MMV 7 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the ICCB-L collection in August 2010. Production of this fungal extract library was funded by a Medicines for Malaria Venture (MMV) grant to Dr. Jon Clardy. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. Crude extracts from the fungi were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • NCDDG 1 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in September 2006. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of 'hit' compounds from extract samples.

  • NCDDG 2 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in May 2007. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of 'hit' compounds from extract samples.

  • NCDDG 3 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in February 2008. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of 'hit' compounds from extract samples.

  • NCDDG 4 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in December 2008. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of 'hit' compounds from extract samples.

  • NCDDG 5 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in January 2009. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of 'hit' compounds from extract samples.

  • NCDDG 6 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in March 2009. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of 'hit' compounds from extract samples.

  • NCDDG 7 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in June 2009. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • NCDDG 8 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in July 2009. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of 'hit' compounds from extract samples.

  • NCDDG 9 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at ~15 mg/ml in DMSO.

    This library was plated and added to the collection in August 2010. Production of this natural product extract library was funded by a National Cooperative Drug Discovery Group (NCDDG) grant from the NIH-NCI to the University of Utah, Harvard Medical School, and the University of British Columbia. The library contains partially purified extracts from endophytic fungi (fungi that live in higher plants) that grow in Costa Rica. The fungi have been collected and assembled by the National Biodiversity Institute (INBio) in Costa Rica. Crude extracts from each organism were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity. This library was assembled under the Harvard Medical School portion of the NCDDG grant.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (Harvard Medical School). Please contact Caroline Shamu to arrange for purification of "hit" compounds from extract samples.

  • NIH Clinical Collection 1 ( Known bioactives collection chemical library )

    384-well Plate format.
    Concentration Information: 10 mM in DMSO.

    The NIH Clinical Collection (NCC) was purchased from BioFocus in Summer 2008 and plated at the ICCBL in October 2009.

    This collection was assembled by the National Institutes of Health (NIH) and is comprised of molecules that have a history of use in human clinical trials.

  • NINDS Custom Collection 2 ( Known bioactives collection chemical library )

    384-well Plate format.
    Concentration Information: 10 mM in DMSO.

    This collection of characterized bioactive compounds was originally compiled by MicroSource Discovery Systems for the National Institute of Neurological Disorders and Stroke (NINDS), the Huntington's Disease Society of America (HDSA), the Amyotrophic Lateral Sclerosis (ALS) Association, and the Hereditary Disease Foundation (HDF). Three quarters of the compounds in the collection are FDA-approved and the rest - which include some controlled substances such as cannabinoids - are known to influence brain activity.

    The current collection (NINDS Custom Collection II) was purchased in 2006 jointly by ICCB-Longwood and the NERCE/NSRB infectious disease screening program.

    For chemical structures and more information about each compound, please go to the ICCB-Longwood Screensaver site.

    Investigators wishing to screen the maximum number of FDA-approved drugs can achieve ~46% coverage (561 compounds) of the FDA set by screening both NINDS and Prestwick. Including the ICCB Biomol Known Bioactives will increase coverage to ~47% of the FDA set, as there is some redundancy among these three libraries.

  • Organic fractions - NCI Plant and Fungal Extracts ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at 5 mg/ml in DMSO.

  • Prestwick1 Collection ( Known bioactives collection chemical library )

    384-well plate format.
    Concentration Information: 2 mg/ml in DMSO.

    The Prestwick library was purchased by the New England Regional Center of Excellence in Biodefense and Emerging Infectious Diseases (NERCE/BEID) in February 2006. This library contains 1120 off-patent compounds that have been selected for structural diversity, collective coverage of multiple therapeutic areas, and known safety and bioavailability in humans. Over 85% of the Prestwick compounds are marketed drugs.

    Investigators wishing to screen the maximum number of FDA-approved drugs can achieve approximately 46% coverage (561 compounds) of the FDA set by screening both NINDS and Prestwick. Including the ICCB Biomol Known Bioactives will increase coverage to ~47% of the FDA set, as there is some redundancy among these three libraries.

    Many of the compounds in the Prestwick library can be obtained from Sigma-Aldrich, but they can also be ordered directly from Prestwick.

  • Qiagen Human 1: miRNA Mimics ( miRNA library )

    384-well plate format.
    Concentration Information: 1uM in buffer.
    Control Information: AllStars Negative Control siRNA (Qiagen Cat#: 1027281); Human PLK1 siRNA SMARTPool (Dharmacon Cat#: M-003290-01)

    This library is the Qiagen Human miScript miRNA Mimic Library and was purchased in the Summer of 2009. The design of this library was based on miRBase release 13.0.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

  • Sigma esiRNA Human 1 ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    ICCBL screeners interested in screening this library should contact Caroline Shamu for more details.

  • Sigma esiRNA Human 2 ( siRNA library )

    384-well plate format.
    Concentration Information: 1uM in siRNA buffer.

    The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.

    ICCBL screeners interested in screening this library should contact Caroline Shamu for more details.

  • Starr Foundation Extracts 2 ( Natural product extracts chemical library )

    384-well plate format.
    Concentration Information: Dried extracts resuspended at 15 mg/ml in DMSO.

    Production of this plant extract library was funded by a grant from the Starr Foundation to the Osher Institute of Medicine at Harvard Medical School. The library contains extracts from plants used in traditional Chinese medicine. Crude extracts from the plants were obtained and further fractionated to eliminate nuisance compounds, especially those of high molecular weight or high polarity.

    Screening positives that have been confirmed by follow-up experiments on cherry picked samples can be further fractionated via a collaboration with the lab of Dr. Jon Clardy (BCMP Department, Harvard Medical School). Please contact Caroline Shamu or Jennifer Smith to arrange for purification of “hit” compounds from extract samples.

    The Starr2 library was created by reformatting many of the same extracts that were present in Starr1. There are no extracts in Starr2 that were not also present in Starr1.

Services

  • High throughput microscopy service ( Material analysis service )

    User-based facility. Equipment and techniques used depend on the project and user needs.

  • Lab automation service ( Material processing service )

    Consultation and assistance with automation of experimental procedures in 96-well, 384-well, and 1536-well plate formats. User-based facility. Equipment and techniques used depend on the project and user needs.

  • Quantitative PCR service ( Material analysis service )

    User-based facility. Equipment and techniques used depend on the project and user needs.

  • RNAi screening service ( Material analysis service )

    ICCB-Longwood provides full-genome human and mouse siRNA and miRNA mimic and inhibitor libraries and staff assistance for designing, conducting, and interpreting high-throughput RNAi screens. Please note that ICCB-L provides RNAi reagents only within the context of a high-throughput screen and does not supply individual siRNAs or miRNAs or subsets of siRNAs or miRNAs for other purposes.

  • Small molecule screening service ( Material analysis service )

    The ICCB-Longwood Screening Facility provides small molecule libraries and staff assistance for designing, conducting, and interpreting high-throughput chemical screens. The ICCB-Longwood compound collection is continuously growing, and over 500,000 compounds are currently available for screening. Please note that ICCB-Longwood provides small molecule reagents only within the context of a high-throughput screen and does not supply individual compounds or subsets of compounds for other purposes.

Software

  • Dotmatics Vortex data ( Software )

    "Vortex is a data visualisation and analysis solution for all industries. As an alternative to spreadsheets, it provides the plots and functionality required to explore and understand any complexity and size of data. Vortex is also available with full chemical structure intelligence."

  • Scitegic Pipeline Pilot ( Software )

    "Pipeline Pilot is very useful for analyzing screening data. It can rapidly analyze large datasets with user-specified computational protocols that are assembled in a modular fashion."

  • Screensaver (laboratory information management system) ( Software )

    Screensaver is a free, open source, web-based lab information management system (LIMS) that supports the storage and comparison of screening data sets generated by small molecule and RNAi screens, as well as the management of information about screens, screeners, libraries, and laboratory work requests.


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Last updated: 2015-12-01T15:43:55.875-06:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016