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DNA Resource Core (DF/HCC)

Director: Mohr, Stephanie, Ph.D.

Location: 240 Longwood Ave, C1-204, Boston, MA 02115

Summary:

The DNA Resource Core was started in the spring of 1999 to meet a growing need for DNA sequencing services at a cost that is affordable for academic labs. Our services now include DNA sequencing for large- and small-scale projects and a plasmid repository & distribution service. Our highest priorities are quality assurance, user support and timely request fulfillment.

Questions about the plasmid repository can also be directed to plasmidhelp@hms.harvard.edu. (Please Note: we have drop-off locations in the Longwood Medical Area and at the Harvard Biolabs. Call for pick-ups at MGH Charlestown or other locations.)

Affiliations:

People:

Resources:

Instruments

  • ABI 3730xl DNA analyzer ( DNA sequencer )

    Lab contains two 96-capillary ABI 3730xl DNA Analyzers. "The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample."

  • Biobank workstation ( Biobank picking station )

    Has parts:
    Biobank;
    Biobank Picking Station

  • Biomek FX ( Liquid handler )

  • Biomek NX ( Liquid handler )

    96-well format

  • BioTRacy Picking Station ( Biobank picking station )

    Attached to both Biobanks; uses 2D barcodes.

  • Hamilton Sample Access Management System ( Biobank picking station )

    A localized storage system used for secure sample management of plates and tubes.

  • Thermo Fisher Life Technologies Veriti® thermal cycler ( PCR instrument )

    Facility has ten Veriti thermal cyclers.

Reagents

  • Assay Specific Vectors ( Construct library )

    Individual vectors used for a particular assay.
    * Viral Production (lentivirus, moloneyvirus, baculovirus)
    * RNA Interference
    * Mammalian Expression
    * Drosophila Expression
    * Bacterial Expression

  • cDNA / ORF Entry Plasmids ( cDNA library )

    Protein coding genes from numerous organisms cloned into entry vectors for storage and cloning purposes.
    * Human Proteome (From the ORFeome Collaboration [OC], Mammalian Gene Collection [MGC], Center for Cancer Systems Biology [CCSB])
    * Mouse Proteome (From the Mammalian Gene Collection [MGC])
    * Yeast Proteome (From the Harvard Institute of Proteomics [HIP])
    * Microorganism Proteomes (From the Harvard Institute of Proteomics [HIP])
    * Drosophila Proteome (From the Drosophila RNAi Screening Center [DRSC])

  • cDNA/ ORF Expression Plasmids ( cDNA library )

    Protein coding genes from numerous organisms cloned into expression vectors for a specific assay.
    * Human Proteome (From the ORFeome Collaboration [OC], Mammalian Gene Collection [MGC], Center for Cancer Systems Biology [CCSB])
    * Yeast Proteome (From the Harvard Institute of Proteomics [HIP])
    * Microorganism Proteomes (From the Harvard Institute of Proteomics [HIP])

  • Focused Libraries ( Construct library )

    Focused collection of constructs for a particular assay or research topic.
    * Human Chemokine (cDNA/ ORF)
    * Human Kinase (cDNA/ ORF)
    * Breast Cancer (cDNA/ ORF)
    * GPCR (shRNA)
    * Kinase (shRNA)
    * Non-protein Kinase (shRNA)
    * Protein Kinase (shRNA)
    * Transcription Factors (shRNA)
    * Tyrosine Kinase (shRNA)
    * pPRIME Cloning (plasmid vectors)
    * MAGIC (plasmid vectors)

  • shRNA Expression Plasmids ( shRNA library )

    shRNA hairpin constructs targeting specific regions of a gene for use in knockdown assays.
    * Human Proteome (The RNAi Consortium [TRC])

Services


Web Links:

Last updated: 2017-03-28T17:01:23.046-05:00

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