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Transgenic Mouse Core (HMS)

Director: Sharpe, Arlene H., M.D., Ph.D.

Location: New Research Building, Rm. 837, 77 Ave. Louis Pasteur, Boston, MA 02115

Summary:

Established in 1992, the Transgenic Mouse Core provides services for the generation of transgenic and knockout mice using state-of-the-art facilities and equipment.

Dr. Lina Du, formerly a plastic surgeon in Beijing and an expert in microvascular surgery, performs both blastocyst and pronuclear injections. She has over 20 years of experience in embryo manipulation and the generation transgenic mice.

The Transgenic Mouse Core has all of the equipment necessary for generation of transgenic mice including a Nikon Diaphot microscope equipped with Nomarski Optics and Narishige micromanipulators for microinjections, Nikon surgical microscopes for egg isolation ad transfer, a sutter needle puller and a de Fonbrune microforge. The facility maintains mice necessary for egg donors, egg recipients, and vasectomized males. This is a non-profit facility and charges are based upon anticipated mouse costs, maintenance of mice and equipment, purchase of necessary surgical supplies and chemicals, and personnel costs.

ES cell culture services have been offered for 15 years. Our staff carries out electroporation of targeting vectors into ES cells and provides investigators with DNA to identify ES cells carrying the desired recombination events. Staff then expand the appropriate ES cells for microinjection into blastocysts by the Transgenic Mouse Core.

In recent years, the core has expanded its list of services to include CRISPR injections and cryopreservation.

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Last updated: 2017-01-05T12:29:17.668-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016