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Tabin Laboratory

Location: 77 Ave Louis Pasteur-NRB360, Boston, MA 02115

Summary:

The laboratory studies the genetic basis by which form and structure are regulated during vertebrate development. We combine classical methods of experimental embryology with modern molecular and genetic techniques for regulating gene expression during embryogenesis.

One of the classic systems for the study of embryonic development is the chick embryo, where grafting experiments have given profound insight into such questions as the patterning of developing limb axes, and the control of organogenesis. These classical experiments provide a context for interpreting modern molecular studies and the methods they employed also give us an additional set of tools for manipulating the embryo. For example, we can use retroviral vectors to alter gene expression in the context of specific transplantations or extirpations. Important complementary information is gained from studies taking advantage of the powerful techniques for regulated misexpression and gene deletion in the mouse.

The lab has major efforts underway exploiting these approaches to understand limb development, from the establishment of the initial axes, to understanding the difference in genetic controls between an arm and a leg, through later specific events such as differential bone growth and specific muscle patterning; and to understand the establishment of left-right asymmetry (e.g.. why your heart is on the left and not the right) from the initiation of the left-right difference, through signaling cascades, to left- or right-specific morphogenesis. We also currently have projects looking at patterning of the gut, the differentiation of the somites and morphogenesis of the heart, as well as biochemical analysis of the hedgehog signal transduction system, a key signaling pathway during development.

Affiliations:

People:

Resources:

Organisms and Viruses

  • BMP2 cKO ( Mus musculus )

    Used to study limb development.

    Conditional gene deletion.

  • BMP2 cKO x BMP4 cKO ( Mus musculus )

    Used to study limb development.

  • BMP2 cKO x BMP4 cKO x Prx1 Cre ER ( Mus musculus )

    Limb bud specific, incucible, BMP2 and BMP4 mutant.

    Used to study limb development.

  • BMP2 cKO x Prx1 Cre ER ( Mus musculus )

    Limb bud specific, inducible.

    Used to study limb development disorder, limb development.

  • BMP4 cKO ( Mus musculus )

    Conditional gene deletion.

    Used to study limb development.

  • BMP4 cKO x Prx1 Cre ER ( Mus musculus )

    Limb bud specific, inducible, BMP4 mutant.

    Used to study limb development disorder, limb development.

  • Dicer cKO ( Mus musculus )

    Conditional gene deletion.

    Used to study limb development.

  • Dicer-cKO x Prx1 Cre ER ( Mus musculus )

    Limb bud specific, incucible, Dicer mutant.

    Used to study limb development, limb development disorder, RNAi function.

  • Fertilized chicken eggs (White Leghorn) ( Gallus gallus )

    Embryos are manipulated by retroviral injection, electroporation, tissue transplantation and tissue labeling.

    Wild type.

  • Prx1 Cre ER ( Mus musculus )

    Limb bud specific inducer of gene deletion.

    Used to study limb development.

  • Rosa Cre ( Mus musculus )

    Ubiquitous inducer of gene deletion.

    Used to study limb development.

  • Scx-GFP ( Mus musculus )

    Mouse has a transgenic insertion of the Scleraxis promoter driving expression of eGFP.

  • Shh Cre-GFP ( Mus musculus )

    ZPA specific inducer of gene deletion and fluorescence.

    Used to study limb development.

Reagents

  • 104d13-10NheI ( Plasmid )

  • 104d13-10RI ( Plasmid )

  • 104d13-11XbaI ( Plasmid )

  • 104d13-5.2RI ( Plasmid )

  • 104d13-6X/B ( Plasmid )

    Intermediate of 3' arm of conditional W14 knockout construct.

  • 104d13-7.0Sm/B ( Plasmid )

    3' arm of lacZ/ki construct

  • 104d13-8.5Xba ( Plasmid )

  • 104d13-8NheI ( Plasmid )

  • 11N15 ( Plasmid )

    From craniofacial array.

  • 12M14 ( Plasmid )

    From craniofacial array.

  • 13G24 ( Plasmid )

    From craniofacial array.

  • 15P20 ( Plasmid )

    From craniofacial array.

  • 2-E24 ( Plasmid )

    From craniofacial array.

  • 21K24 ( Plasmid )

    From craniofacial array.

  • 29M20 ( Plasmid )

    From craniofacial array.

  • 32P14 ( Plasmid )

    From craniofacial array.

  • 36K18 ( Plasmid )

    From craniofacial array.

  • 37B3 ( Plasmid )

    From craniofacial array.

  • 37L24 ( Plasmid )

    From craniofacial array.

  • 3I2 ( Plasmid )

    From craniofacial array.

  • 3O22 ( Plasmid )

    From craniofacial array.

  • 42C19 ( Plasmid )

    From craniofacial array.

  • 45D9 ( Plasmid )

    From craniofacial array

  • 45M14 ( Plasmid )

    From craniofacial array.

  • 46D5 ( Plasmid )

    From craniofacial array.

  • 49I24 ( Plasmid )

    From craniofacial array.

  • 51M10 ( Plasmid )

    From craniofacial array.

  • 53P14 ( Plasmid )

    From craniofacial array.

  • 53P23 ( Plasmid )

    From craniofacial array.

  • 54-E18 ( Plasmid )

    From craniofacial array.

  • 6J16 ( Plasmid )

    From craniofacial array.

  • 9J10 ( Plasmid )

    From craniofacial array.

  • 9J12 ( Plasmid )

    From craniofacial array.

  • AP Tag 4 ( Plasmid )

    Expression CMV SV40

  • BSmSCX ( Plasmid )

    Missing the first 5 amino acids. Scleraxis is expressed in early developing and in adult tendons and ligaments.

  • C3-transferase-RCAS(A) ( Plasmid )

    Retroviral misexpression. Toxic for DF-1 cells.

  • cAgg pGEM-Te (#23) ( Plasmid )

    In situ probe.

  • Cbfa-1 (I-2) ( Plasmid )

    In situ probe.

  • cCD44rel pGEM-Te ( Plasmid )

    In situ probe.

  • cDkk 1 ( Plasmid )

    In situ probe.

  • cErm ( Plasmid )

  • cEya2Slax ( Plasmid )

    Template for TNT rexns in GST pull-down experiments.

  • cfgf 8 RCAS (B) ( Plasmid )

  • cFrzB-1/pSlax13 ( Plasmid )

    RCAS cloning intermediate and in situ probe.

  • cFz2 fl. pBst ( Plasmid )

  • cGDF5 pGEMTe (#6) ( Plasmid )

    In situ probe.

  • cGli2 ( Plasmid )

    In situ probe.

  • cGrg 4 ( Plasmid )

    In situ probe.

  • ChEST111M4 ( Plasmid )

  • ChEST126J12 ( Plasmid )

  • ChEST147a8 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST167i10 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR. Genbank Accesion # AB033634, AB033633, X85731.

  • ChEST177b22 ( Plasmid )

    In situ probe.

  • ChEST177e21 ( Plasmid )

    in situ probe

  • ChEST21A20 ( Plasmid )

  • ChEST223D3 ( Plasmid )

  • ChEST257g24 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST260d4 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST27k22 ( Plasmid )

    In situ probe. PCR doesn't work to linearize.

  • ChEST280l8 ( Plasmid )

    In situ probe.

  • ChEST339j24 ( Plasmid )

    In situ probe.

  • ChEST357a21 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST35i24 ( Plasmid )

    To linerarize - use M13 F / R PCR. Genbank Accesion # AF074605. In situ probe.

  • ChEST363l11 ( Plasmid )

    In situ probe. PCR doesn't work to linearize.

  • ChEST364f11 ( Plasmid )

    In situ probe.

  • ChEST376N5 ( Plasmid )

  • ChEST404l3 ( Plasmid )

    In situ probe.

  • ChEST421b10 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST439a8 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST460B19 ( Plasmid )

  • ChEST48g17 ( Plasmid )

    In situ probe.

  • ChEST49L2 ( Plasmid )

    10d embryo cDNA, RIKEN FULL-LENGTH ENRICHED LIBRARY, CLONE:2610528H19, FULL INSERT SEQUENCE.

  • ChEST501f14 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST507n16 ( Plasmid )

    In situ probe.

  • ChEST513c7 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST513f16 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST514o7 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST516g22 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST518d19 ( Plasmid )

    To linearize - use M13 F / R PCR. In situ probe.

  • ChEST519c15 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST564a12 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR. Genbank Accesion # AF181680, AF064253.

  • ChEST56h19 ( Plasmid )

    To linerarize - use M13 F / R PCR. Genbank Accesion # AF124511. In situ probe.

  • ChEST607H8 ( Plasmid )

  • ChEST635K12 ( Plasmid )

  • ChEST650k18 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR. Genbank Accesion # AF181680, AF064253.

  • ChEST656017 ( Plasmid )

    In situ probe.

  • ChEST66n9 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST679H19 ( Plasmid )

  • ChEST67o2 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST698n16 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST71f6 ( Plasmid )

    In situ probe.

  • ChEST752i6 ( Plasmid )

    In situ probe.

  • ChEST758N24 ( Plasmid )

  • ChEST764K22 ( Plasmid )

  • ChEST76f23 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST78b10 ( Plasmid )

    In situ probe.

  • ChEST798p11 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR. Genbank Accesion # AF262935.

  • ChEST800n13 ( Plasmid )

    To linearize - use M13 F / R PCR. In situ probe.

  • ChEST80e16 ( Plasmid )

  • ChEST810h13 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR. Genbank Accesion # AF262935.

  • ChEST831A15 ( Plasmid )

    Linearize with PCR. In situ probe.

  • ChEST858m21 ( Plasmid )

  • ChEST861e19 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST870g21 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR. Genbank Accesion # AB033634, AB033633, X85731.

  • ChEST903j23 ( Plasmid )

  • ChEST909l22 ( Plasmid )

    In situ probe.

  • ChEST921k6 ( Plasmid )

    In situ probe. To linerarize - use M13 F / R PCR.

  • ChEST949L9 ( Plasmid )

  • ChEST966i4 ( Plasmid )

    In situ probe.

  • ChEST968e6 ( Plasmid )

    In situ probe.

  • ChEST973m8 ( Plasmid )

    In situ probe.

  • Chfz1[delta]C RCAS(A) ( Plasmid )

  • Chfz1[delta]C RCAS(B) ( Plasmid )

  • Chfz1wt RCAS(A) ( Plasmid )

  • Chfz1wt RCAS(B) ( Plasmid )

  • Chfz7[delta]C RCAS(A) ( Plasmid )

  • Chfz7wt RCAS(A) ( Plasmid )

  • Chfz7wt RCAS(B) ( Plasmid )

  • cHlx ( Plasmid )

    In situ probe.

  • cHox A10n ( Plasmid )

    In situ probe.

  • cHox A11 ( Plasmid )

  • cHox A11m ( Plasmid )

    In situ probe.

  • cHox A13s ( Plasmid )

    In situ probe.

  • cHox A4a ( Plasmid )

    In situ probe. Published name - Chox 1.4 Accession # x52670.

  • cHox A9a ( Plasmid )

    In situ probe. Published name - Chox 1.7.

  • cHox B4 ( Plasmid )

    Published name - Chox-z Accession # x17612. In situ probe.

  • cHox B8b ( Plasmid )

    In situ probe. Full-length published sequence - accession # U81801. Published Name - Chox2.4; Accession # x52748.

  • cHox B8d ( Plasmid )

    In situ probe.

  • cHox B8e ( Plasmid )

    In situ probe.

  • cHox B9 ( Plasmid )

    Different from #792 which is also chick Hox B9.

  • cHox B9 ( Plasmid )

    in situ probe

  • cHox C10 or HCC10 ( Plasmid )

  • cHox C10d ( Plasmid )

    In situ probe.

  • cHox C11 ( Plasmid )

  • cHox C11f ( Plasmid )

    In situ probe.

  • cHox C4 or CHC4* ( Plasmid )

  • cHox C4c ( Plasmid )

    In situ probe.

  • cHox C5 ( Plasmid )

  • cHox C5m ( Plasmid )

    In situ probe.

  • cHox C6 RCAS ( Plasmid )

    Viral misexpression.

  • cHox C6 Slax ( Plasmid )

  • cHox C6h ( Plasmid )

    In situ probe.

  • cHox C6H8 ( Plasmid )

  • cHox C8 or CHC8* ( Plasmid )

  • cHox C8g ( Plasmid )

    In situ probe.

  • cHox C9 or CHC9 ( Plasmid )

  • cHox C9c ( Plasmid )

    In situ probe.

  • cHox D10 ( Plasmid )

    Published name - Chox 4.5 4d.

  • cHox D10 RCAS ( Plasmid )

    Published name - Chox 4.5 4d.

  • cHox D11 or CHD11 ( Plasmid )

  • cHox D11a ( Plasmid )

    In situ probe. Published Name - Ghox 4.6 4e Accession # - CHKHOXA.

  • cHox D12a ( Plasmid )

    In situ probe. Published Name - Ghox 4.7 4f Accession # - GGGHOX.

  • cHox D13 ( Plasmid )

  • cHox D13dd ( Plasmid )

    Published Name - d-13 4.8 4g Accession # - L09550. **when blast seq - got mHox D9 - NOT SURE IF D13!!! In situ probe.

  • cHox D13ks1 ( Plasmid )

    In situ probe.

  • cHox D13x ( Plasmid )

  • cHox D13x RCAS ( Plasmid )

    Viral misexpression

  • cHox D4 ( Plasmid )

    Published name - Chox-a (accession # x52672). In situ probe.

  • cHox D8h ( Plasmid )

    In situ probe. Published Name - Chox-m Accession # - CHCHOXM.

  • cHox D9 ( Plasmid )

  • cHox D9zi ( Plasmid )

    In situ probe.

  • cInv ( Plasmid )

    In situ probe, library probe.

  • cIrx 4 ( Plasmid )

  • cIrx 5 ( Plasmid )

  • cIrx 6 ( Plasmid )

  • cKO W14 ( Plasmid )

    Conditional knock-out construct.

  • cMFH 1 - pBS ( Plasmid )

    In situ probe.

  • cMkp3 ( Plasmid )

    In situ probe.

  • cNkx 2.5 RCAS (A) ( Plasmid )

    Retroviral misexpression.

  • cNkx 2.5 RCAS (B) ( Plasmid )

    Retroviral misexpression.

  • cParaxis ( Plasmid )

    In situ probe.

  • cPea 3 ( Plasmid )

    In situ probe.

  • CPV-2 ( Plasmid )

  • cRhoA-pGEM ( Plasmid )

    In situ probe.

  • cSef ( Plasmid )

    In situ probe.

  • cSim1 - pGem ( Plasmid )

    Linearize with PCR. In situ probe.

  • cSu(fu) pBS SK- (RPD 5) ( Plasmid )

  • cW14 pGem-Teasy ( Plasmid )

    In situ probe.

  • cW14-RCASBP(A) ( Plasmid )

    Retroviral misexpression.

  • cWnt-5b Slax13 ( Plasmid )

    Cloning intermediate / in situ probe.

  • cWnt13-pGEMTe ( Plasmid )

    In situ probe.

  • cWnt5b-RCAS ( Plasmid )

    Retroviral misexpression.

  • cZic1 ( Plasmid )

    In situ probe, library probe.

  • cZic2 ( Plasmid )

    In situ probe, library probe.

  • cZic3 ( Plasmid )

    In situ probe, library probe.

  • Dicer ES cell target conditional ( Plasmid )

    Sequenced. Targets RNA3a domain of Dicer.

  • Dicer ES cell target null ( Plasmid )

    Targets RNA3b. Sequenced.

  • dn FGF R1 - RCAS (A) ( Plasmid )

  • dn FGF R1 - RCAS (B) ( Plasmid )

  • dnFREK - RCAS (A) ( Plasmid )

  • dnFREK - RCAS (B) ( Plasmid )

  • dnFREK - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • DNR Lac Z ( Plasmid )

    pBK CMV with Lac Z.

  • DRhoN19-RCAS(A) ( Plasmid )

    Retroviral misexpression.

  • DRhoV14-RCAS(A) ( Plasmid )

    Retroviral misexpression.

  • DsR-EGFP-N1 ( Plasmid )

    Expression of lab EGFP (from Nolan) under control of N1 vector (for comparison to DsRed 1 and 2 from Clontech).

  • Ecotropic Helper ( Plasmid )

    Helper plasmid for packaging viruses from constructs containing LTRs by cotransfection. Also useful for boosting titer from phoenix packaging cells by cotransfection with viral source plasmid (5μgs ecotropic helper with 15μgs viral construct for 10cm plate).

    There is no sequence or vector map information for this construct (but it does work).

  • GG3gR2 ( Plasmid )

    To analyze the state of Gli3 cleavage in living cells that are responding to Shh.

  • GG3R1-N1 ( Plasmid )

    To analyze the state of Gli3 cleavage in living cells that are responding to Shh.

  • GG3R2 ( Plasmid )

    To analyze the state of Gli3 cleavage in living cells that are responding to Shh.

  • GI Vector ( Plasmid )

  • hGdf11-RCAS (A) ( Plasmid )

  • hGdf11-Slax ( Plasmid )

  • hGli 1 pcDNA3 ( Plasmid )

    Vector has a pCMV promoter.

  • hGli3-Red1-N1 ( Plasmid )

    To analyze the state of Gli3 cleavage in living cells that are responding to Shh.

  • lacZkiW14 ( Plasmid )

    LacZ knock-in/knock-out construct for Wnt14 locus.

  • LmxRISAP ( Plasmid )

  • LmxSlires11 ( Plasmid )

  • mATX (EST 00016397) ( Plasmid )

    The Est is cloned as an RI, NotI fragment but there is also an internal RI site. Sequence is in mATX.

  • mBMP 2 del probe ( Plasmid )

    Sequenced. In situ probe, for detecting floxed BMP 2 allele.

  • mBMP 4 del probe ( Plasmid )

    In situ probe for detecting floxed BMP 4 allele.

  • mCollagen XII-pBS SK ( Plasmid )

    In situ probe

  • mDach 2 ( Plasmid )

    Probe for ISH.

  • mDicer full length cDNA ( Plasmid )

    Sequenced.

  • mDkk 1 - RCAS (A) ( Plasmid )

  • mDkk 1 - RCAS (B) ( Plasmid )

  • mDkk 1 - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mDll1 v.2 RCAS (A) ( Plasmid )

    Retroviral misexpression.

  • mE12 - RCAS (B) ( Plasmid )

  • mE12 - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mE47 - RCAS (B) ( Plasmid )

  • mE47 - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mFGF R4 ( Plasmid )

    In situ probe. Genbank Accesion # 5043727.

  • mGrg 1 ( Plasmid )

    In situ probe.

  • mGrg 3a ( Plasmid )

    In situ probe.

  • mGrg 3b ( Plasmid )

    In situ probe.

  • mGrg 4 ( Plasmid )

    In situ probe.

  • mGrg 5 ( Plasmid )

    In situ probe.

  • mHlx - RCAS (A) ( Plasmid )

    Internal Cla site removed by PCR.

  • mHlx - RCAS (B) ( Plasmid )

    Internal Cla site removed by PCR.

  • mHlx - Slax ( Plasmid )

    Linearize with PCR. In situ probe. Internal Cla site removed by PCR.

  • mHox A11 ( Plasmid )

    In situ probe.

  • MHox Cre Trng ( Plasmid )

    Transgenic construct.

  • mHox D11 ( Plasmid )

    In situ probe.

  • mHox D11 RCAS (A) ( Plasmid )

    Retrovirus production.

  • MHox Pitx1 Trng ( Plasmid )

    Transgenic construct.

  • MHox Tbx4 Trng ( Plasmid )

    Transgenic construct.

  • MHox Tbx5 Trng ( Plasmid )

    Transgenic construct.

  • MHox-Eck transgenic construct ( Plasmid )

    Linearize with Xba I, Sal I. Transgenic mice.

  • MHox-Gal4 Transgenic Construct ( Plasmid )

    Linearize w/ Not I, Kpn I. Transgenic mice.

  • MHox-trEck transgenic construct ( Plasmid )

    Linearize w/ Xba I, Sal I. Transgenic mice.

  • mImfa - RCAS (A) ( Plasmid )

  • mImfa - RCAS (B) ( Plasmid )

  • mImfa - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mMyf 5 - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mMyo R - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mMyoD - Slax ( Plasmid )

    In situ probe.

  • mPax 1 - pBS ( Plasmid )

    In situ probe.

  • mPax 1 - RCAS (A) ( Plasmid )

  • mPax 1 - Slax ( Plasmid )

    In situ probe.

  • mPax 1 T EnR - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mPax 1 T VP16 - Slax ( Plasmid )

  • mPax 3- Slax ( Plasmid )

    Linearize with PCR. In situ probe. Internal Cla site removed by PCR.

  • mPax 9 - pBS ( Plasmid )

    In situ probe.

  • mPDGFA - RCAS (A) ( Plasmid )

  • mPDGFA - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mPea3-Slax ( Plasmid )

    Shuttle vector for RCAS or in situ hybridization. Generate probe template with M13F and M13R PCR.

  • mScx - RCAS (A) ( Plasmid )

  • mScx - RCAS (B) ( Plasmid )

  • mScx - Slax ( Plasmid )

    Linearize with PCR. In situ probe.

  • mScx5p ( Plasmid )

    To clone any ORF at start of Scx.

  • mScxAP ( Plasmid )

    Transgenic construct for expression of AP under control of Scx regulatory elements.

  • mScxCre ( Plasmid )

    Targeting vector.

  • mScxLuc ( Plasmid )

    Targeting Vector.

  • msfrp3-RCAS(A) ( Plasmid )

    Retroviral misexpression. Have not observed a phenotype.

  • mSix1-Slax ( Plasmid )

    Backbone constructed from Guillermo Oliver's RCAS-Six 1 clone RCAS-Six 1 / Cla I into Slax / Cla I. Template for TNT reactions in GST pull-down experiments.

  • mSmo-R1E5 ( Plasmid )

    Track accumulation and localization of Smoothened protein.

  • mSmo-Red2 ( Plasmid )

    Track accumulation and localization of Smoothened protein.

  • mTendin ( Plasmid )

    In situ probe.

  • mW4 RCAS(A) ( Plasmid )

  • mWnt14-550 ( Plasmid )

    In situ probe.

  • p RSCH or p RCS ( Plasmid )

    In situ probe for detecting RCAS virus.

  • p10Dhhz ( Plasmid )

  • p1367 ( Plasmid )

  • p15f ( Plasmid )

    Randy Johnson's library (NG wing+leg) isolate; NOT full length- truncated at 3' end.

  • p19a1.5 ( Plasmid )

    In situ probe

  • p3b:2 ( Plasmid )

    In situ probe.

  • pAct R IIa ( Plasmid )

  • pAct R IIb ( Plasmid )

  • pACT2-cEya 2 ( Plasmid )

    Activation domain construct.

  • pActivin ba ( Plasmid )

  • pActivin bb ( Plasmid )

  • pAS2-1 cDach 2 ( Plasmid )

    Yeast 2-hybrid DNA binding construct. pAS2-1 / Nco I + 550 bp 5' Nco I frag of Dach2, pAS2-1 + 5' Nco I Dach 2 frag / Sal I + Dach2 / Sal I 3' fragment.

  • pAS2-1 cEya 2 ( Plasmid )

    DNA binding construct.

  • pAS2-1 cSix 3 ( Plasmid )

    DNA binding construct

  • pAS2-1 mSix 1 domain ( Plasmid )

    Yeast 2-hybrid DNA binding construct. Eco RI digest of Guillermo Oliver's pBTMSix 1 into pAS2-1 Eco RI site.

  • Pax1 T EnR - RCAS (A) ( Plasmid )

  • Pax1 T EnR - RCAS (B) ( Plasmid )

  • Pax1 T VP16 - RCAS (A) ( Plasmid )

  • Pax1 T VP16 - RCAS (B) ( Plasmid )

  • pb-catenin RCAS A ( Plasmid )

  • pbArgos ( Plasmid )

  • pbAristaless ( Plasmid )

  • pBCLXL 1 ( Plasmid )

  • pbDac ( Plasmid )

  • pBK AP ( Plasmid )

    pBluescript with Alkaline Phosphatase.

  • pBK CMV -Shh (pN 12) ( Plasmid )

  • pBK H Tag ( Plasmid )

  • pBK TR ( Plasmid )

  • pBMP 14 ( Plasmid )

  • pBMP 18 ( Plasmid )

  • pBMP 19 ( Plasmid )

  • pBMP 34 ( Plasmid )

  • pBMP6 ( Plasmid )

  • pBMPRIB 3'UTR ( Plasmid )

  • pBMPRIB 5'UTR ( Plasmid )

  • pBS / Dach 1 #6 ( Plasmid )

  • pBS Apterous ( Plasmid )

  • pBS Foll ( Plasmid )

  • pBS tRNA ( Plasmid )

  • pBS-Car ( Plasmid )

  • pBS-COUPTF-II ( Plasmid )

  • pBS-D3 ( Plasmid )

    Probe synthesis.

  • pBS-osteocalcin ( Plasmid )

  • pBS-rpS6 ( Plasmid )

    From craniofacial array.

  • pBS-Tra ( Plasmid )

    Probe synthesis.

  • pBSK+mERtm ( Plasmid )

    This insert can be used for generating tamoxifen-inducible fusion proteins. This hormone-binding domain of the estrogen receptor contains a mutation (G525R) so that it fails to bind its naturally occurring ligand at normal concentrations but retains a high affinity for the synthetic ligand, 4-hydroxytamoxifen.

  • pBSK-Sugar Crisp ( Plasmid )

    In situ probe.

  • pcBar XI ( Plasmid )

  • pcBarX2b ( Plasmid )

  • pcBarXII ( Plasmid )

  • pcbrn-like 15-3 ( Plasmid )

    Isolate from st.12-15 library.

  • pcBSPII ( Plasmid )

    In situ probe.

  • pcCar full ( Plasmid )

  • pcCar PCR ( Plasmid )

    In situ probe.

  • pcCaronte RCAS ( Plasmid )

  • pcCi NL VP16 ( Plasmid )

    BamHI site between VP16 and Ci Znf.

  • pcCi VP16 RCAS ( Plasmid )

    Ci is a Nco - BamHI fragment, with BamHI between VP16 at 3' end.

  • pcCoco Crisp ( Plasmid )

  • pcCoco Crisp ( Plasmid )

  • pcD 1b ( Plasmid )

    In situ probe.

  • pcD 2c ( Plasmid )

    In situ probe

  • pcd2L ( Plasmid )

  • pcDlx 3 RCAS (A) ( Plasmid )

  • pcDlx 3 RCAS (B) ( Plasmid )

  • pcDNA 3.1 (-) Car-myc ( Plasmid )

  • pcDNA3 cSu(fu) ( Plasmid )

  • pCdx A ( Plasmid )

    In situ probe.

  • pCek 8HK ( Plasmid )

  • pcEN 1 ( Plasmid )

  • pcEPg ( Plasmid )

  • pcEsc B347 ( Plasmid )

  • pcEsc B367 ( Plasmid )

  • pcEsc B384 ( Plasmid )

  • pcFgf10 ( Plasmid )

    In situ probe (replaces probe from Okuchi et al.)

  • pcFGFR1-C1 ( Plasmid )

  • pcFGFR2-B2 ( Plasmid )

  • pcFGFR3-A1 ( Plasmid )

  • pcFu (Kd) Slax #5 ( Plasmid )

  • pcFu 9-1 ( Plasmid )

    Clone pulled from a chick limb bud (st18-24) random primed library; screen was done with 3' RACE 700-8 probe. Nco I ~100bp into clone.

  • pcFu M-AG ( Plasmid )

  • pcFu(G) 2.2 #2 ( Plasmid )

  • pcfu1-800myc-his ( Plasmid )

    Expressed protein is 33 kDa. Expression in epitope tagged chick Fused protein in eukaryotic cells.

  • pcFz-1, full length ( Plasmid )

  • pcFz-1, partial ( Plasmid )

  • pcFz-2, partial 3' ( Plasmid )

  • pcFz-2, partial 5' ( Plasmid )

  • pcFz-7, full length ( Plasmid )

  • pcGli ( Plasmid )

  • pcGli NL VP16 ( Plasmid )

  • pcGli 2 ( Plasmid )

    In situ probe.

  • pcGli 3b-13b ( Plasmid )

    Linkers are [RI,BamHI,KpnI,NcoI]; cloned from c12-15 dT primed library.

  • pcGli 3b-17a ( Plasmid )

    Linkers are [RI,BamHI,KpnI,NcoI]; cloned from c12-15 dT primed library.

  • pcGli 3b-19a ( Plasmid )

    Linkers are [RI,BamHI,KpnI,NcoI]; cloned from c12-15 dT primed library.

  • pcGli 3b-gb1.7 ( Plasmid )

    Linkers are [RI,BamHI,KpnI,NcoI]; cloned from c12-15 dTprimed library.

  • pcGli VP16 RCAS ( Plasmid )

    Gli is a Nco - BamHI fragment, with BamHI between VP16 at 3' end.

  • pcH2AX ( Plasmid )

  • pcH2XR ( Plasmid )

  • pcHH B ( Plasmid )

  • pCI-VSV ( Plasmid )

    Vector for producing G coat virus; contains CMV IE enhancer/promotor; intron; VSV-G; SV40 polyA. Parental vector is pCI from Promega. HIGH copy; VSV from pME-VSV

  • pcIhh R-1-3 Sma ( Plasmid )

  • pcIhh R4-1 Sac ( Plasmid )

    In situ probe.

  • pcIhh R4-1-2-2 ( Plasmid )

  • pcIhh RCAS (A) ( Viral plasmid )

  • pcIhh RCAS (E) ( Viral plasmid )

  • pck-zic3 ( Plasmid )

    In situ probe. Suitable restriction sites at 5' end: BamHI; Xba I; Not I; or Sac I; at 3? end: Xho I; Kpn I.

  • pck-zic3 (#6.C) ( Plasmid )

  • pck-zic3 (#3.C) ( Plasmid )

  • pcl 11 ( Plasmid )

    Insulator plasmid. SEQUENCED - 6/14/01.

  • pcLefty 1 ( Plasmid )

  • pcLmo4 ( Plasmid )

    In situ probe.

  • pcLmx #3 ( Plasmid )

  • pcLmx #5 ( Plasmid )

  • pcMsx 1 (Hx 7s) ( Plasmid )

  • pcMsx 2 (Hox 8) ( Plasmid )

  • pCMX ( Plasmid )

    CMV promoter.

  • pcN-cadherin ( Plasmid )

  • pcNkx 2.5 ( Plasmid )

  • pcNot ( Plasmid )

  • pcNotch 1 ( Plasmid )

  • pcNR -1 ( Plasmid )

  • pcNR-1 RCAS (A) ( Plasmid )

  • pCol II-Ihh ( Plasmid )

    Ihh under Col II promoter expresses high; NO bones; lethal (Andy McMahon).

  • pCol X- Ihh ( Plasmid )

    Low expressing construct in transgenic mice.

  • pCol2-410 ( Plasmid )

    In situ probe.

  • pCON3 ( Plasmid )

    CMV promoter and SV40 poly A tail.

  • pCON3 AP2 ( Plasmid )

    Construct is 6594bp.

  • pcOP ( Plasmid )

    In situ probe.

  • pcPax 3 ( Plasmid )

  • pcPitx 1 RCAS (A) ( Plasmid )

    Insert is NcoI (start) to EcoRI (stop).

  • pcPitx 1 RCAS (B) ( Plasmid )

    Insert is NcoI (start) to EcoRI (stop).

  • pcPitx 2 Flu RCAS(A) ( Plasmid )

    3x Flu tag at 3' end before stop (Nco I-EcoRI insert; EcoRI-BamHI flu tag) use Nco I for diagnostic digest of insert orientation.

  • pcPLZF ( Plasmid )

  • pcPtc 1 ( Plasmid )

  • pcPtc 1 clone 200 ( Plasmid )

  • pcPtc 2 ( Plasmid )

    In situ probe. Additional method for in situ probe is to PCR amplify with M13 fwd and rev primers for 30 cycles (anneal at 52 degrees, and use a 50 ul rxn mix). Use 4ul of PCR mix and run off with T3 or for sense probe with T7. SEQUENCED - 6/14/01.

  • pcPtc Loop 1 + 2 RD67 HA ( Plasmid )

  • pcPtc Loop 1 RD67 HA ( Plasmid )

  • pcPtc Loop 2 RD67 HA ( Plasmid )

    Xenopus expression vector

  • pcPtc RD67 HA ( Plasmid )

  • pcPTHrP ( Plasmid )

  • pcPTHrP - 599 ( Plasmid )

    In situ probe.

  • pcPTHrP receptor ( Plasmid )

  • pCQR 11 RCAS A ( Plasmid )

  • pCQR 11 RCAS B ( Plasmid )

  • pCS enrep ( Plasmid )

    pCS2+ vector with the engrailed repressor domain. Vector is ~4.1kbp.

  • pCS enrep Nkx 2.3 HD ( Plasmid )

  • pCS enrep Nkx 2.5 HD ( Plasmid )

  • pCS2+/Lef1-VP16 ( Plasmid )

  • pCS2+/Lef∆N-bcat ( Plasmid )

  • pCS2+/Lef∆N-bCTA ( Plasmid )

  • pCS2+/Lef∆N-VP16 ( Plasmid )

  • pCS2+/Lef∆N∆HMG-bcat ( Plasmid )

  • pcShh RCAS ( Plasmid )

    Replication competent virus.

  • pcShh RCAS (E) ( Viral plasmid )

    Replication competent virus.

  • pcSlug ( Plasmid )

    5' primer converts 2nd codon from CCA (pro) to GCA (ala) in order to generate a Nco I site; 3' primer is WT plus EcoRI site.

  • pcSmo ( Plasmid )

  • pcSnR ( Plasmid )

  • pcSox2 ( Plasmid )

    In situ probe for the endoderm rostral to the stomach.

  • pcSu(fu) pGex ( Plasmid )

    GST fusions.

  • pcSu(fu)myc-His ( Plasmid )

    Expression of tagged Su(fu) in eukaryotic cells. Expressed protein is 57kDa.

  • pcSugar Crisp ( Plasmid )

  • pcTbx 15 ( Plasmid )

  • pcTCF4.1 ( Plasmid )

    Isoform A of TCF-4 generated by degenerate RT-PCR differs from isoform B in two regions. In situ hybridization probe.

  • pcWnt 11 RCAS (A) ( Plasmid )

  • pcWnt 4 ( Plasmid )

  • pcWnt 7a ( Plasmid )

  • pcWnt 8c ( Plasmid )

  • pcwnt10a ( Plasmid )

  • PCWNT2 ( Plasmid )

  • pD EM 2 ( Plasmid )

  • pDac 38180 human EST ( Plasmid )

  • pDANr #3 ( Plasmid )

  • Pea3EnR-RCASA ( Plasmid )

    Viral misexpression.

  • Pea3FL-RCASA ( Plasmid )

    Viral misexpression.

  • pED ATX (GW39) ( Plasmid )

    pED is the vector used in the Genetics Institute signal peptide trap screen. All inserts were cloned between an RI and a NotI site.

  • pED GW48 ( Plasmid )

    GW48 has a broad mesenchymal expression with very distinct patterns. Highly identical human ESTs were found in the data base. pED is the vector used in the Genetics Institute signal peptide trap scereen. All inserts were cloned between an RI and a NotI site.

  • pED NHAB100 ( Plasmid )

    Expressed in the somites, in specific muscles, mostly dorsal and in additional specific limb mesenchymal domains. pED is the vector used in the Genetics Institute signal peptide trap screen. All inserts were cloned between an RI and a NotI site.

  • pED NHAB163 ( Plasmid )

    Specific expression in the aortic arch, in specific limb muscles and some other mesenchymal domains, possibly some of the tendons. In addition a unique interdigital stripe is expressing. pED is the vector used in the Genetics Institute signal peptide trap scereen. All inserts were cloned between an RI and a NotI site.

  • pED NHAB4 ( Plasmid )

    NHAB4 is a novel gene. The ORF is very small. NHAB4 was expressed in the dorsolateral neural tube, in the myotome and in differentiating skeletal elements. pED is the vector used in the Genetics Institute signal peptide trap scereen. see attached map. All inserts were cloned between an RI and a NotI site.

  • pEF 1 ( Plasmid )

    Chick EF 1a protection probe.

  • pEF 1a ( Plasmid )

  • pFGD 22 (pcGli 2) ( Plasmid )

  • pFgf 2 ( Plasmid )

  • pFgf 2a 1-3 ( Plasmid )

  • pFLAG Car ( Plasmid )

  • pFLAG-Car ( Plasmid )

  • pFNS ( Plasmid )

    fu(1-800) translation gives 33kDa protein. Su(fu) translation gives 57kDa protein. Translation from NRF-IRES in 293T cells produces doublet. Testing NRF-IRES in different cell lines.

  • pFr 10 ( Plasmid )

  • pFr 8 ( Plasmid )

  • pGem mtcf4 ( Plasmid )

    In situ probe. Sequenced.

  • pGEM-cAlk2 ( Plasmid )

    In situ probe.

  • pGEM-cScrb1 ( Plasmid )

    In situ probe. Cloned by RT-PCR from HH stage 5 cDNA pool using primers designed against regions conserved between mouse, human, and zebrafish Scrb1 sequences (vertebrate homologs of Drosophila Scribble, a PDZ-domain containing protein involved in epithelial polarity).

  • pGEM-cSmad5 ( Plasmid )

    Insert contains one internal EcoRI site. In situ probe.

  • pGEM-EasyT-cVangl1 ( Plasmid )

    In situ probe. Vangl1 is a vertebrate homolog of the Drosophila tissue polarity gene Strabismus/Van Gogh. Also called Stbm.

  • pGEM-T/cW14 ( Plasmid )

    In situ probe. Vector is manufactured by Promega.

  • pGEM-Teasy-cLrdr ( Plasmid )

    In situ probe.

  • pGex 4T-3 modified ( Plasmid )

    Added Cla I and Nco I site in frame. Modified to allow direct cloning from Slax shuttle vectors. Used to make pGEX-GST fusions.

  • pGex Pitx1 N/R ( Plasmid )

    Used for GST fusion experiments. EcoRI site sits directly after endogenous stop codon.

  • pGEX-KG ( Plasmid )

    Cloning vector for GST - pulldown experiments.

  • pGEX-KG + 5' Dach2 frag ( Plasmid )

    GST - pulldown experiments.

  • pGEX-KG + Eya 2 ( Plasmid )

    GST - pulldown experiments.

  • pGEX-KG Dach2 ( Plasmid )

    516 bp Dach2 5' NcoI frag cloned into pGEX-KG opened with NcoI to create pGEX-KG + 5' Nco frag Dach2. GST pull-down experiments. pGEX-KG + 5'Nco frag Dach2 opened with SalI, removing a 304 bp SalI-NcoI fragment. 1835 bp Dach2 3?SalI fragment cloned into pGEX-KG + 5' Nco frag Dach2 to create pGEX-KG Dach2.

  • pGFP CS2+ ( Plasmid )

    Expression vector with CMV 1E94 promoter. Expresses GFP.

  • pGFP RCAS ( Plasmid )

    Control for viral infections. Expresses marker: GFP. Xba I- Nco I fragment moved into Slax 13 at Sma I -Nco I site; the cut out with Cla I and cloned into RCAS (A) Cla I.

  • pGT-A2 ( Plasmid )

  • phGli 3 rg1 ( Plasmid )

  • phGli3 5'-Pst I ( Plasmid )

  • phGli3 5?-Cla I ( Plasmid )

  • phGli3 BamHI-Hind III ( Plasmid )

  • phGli3 Cla I/Hind III ( Plasmid )

  • phGli3 Swa/HIII ( Plasmid )

  • phGli3- Xmn1 ( Plasmid )

    For use in mapping Gli 3 interaction domain. Xmn I / EcoRV killed site.

  • pHH 10D ( Plasmid )

  • pHH 2b ( Plasmid )

  • phIhh ( Plasmid )

  • pHNF 12 ( Plasmid )

  • pHox C10 ribovector ( Plasmid )

  • phShh ( Plasmid )

  • phSmo M1 RCAS (B) ( Plasmid )

    Replication competent virus.

  • phSmo M2 RCAS (B) ( Plasmid )

    Replication competent virus.

  • phSmo WT RCAS (B) ( Plasmid )

    Replication competent virus.

  • pHumanSugarCrisp ( Plasmid )

  • pIhh poly A ( Plasmid )

  • pIhh R-2-2 PB ( Plasmid )

  • pJA1227 ( Plasmid )

  • pJA1300 / mPtx1DC ( Plasmid )

  • pJA1301 / mPtx1 DN ( Plasmid )

  • pJA907 / mPtx 1 WT ( Plasmid )

    The Kpn I site is unique.

  • pL RCAS (A) ( Plasmid )

  • pL RCAS (B) ( Plasmid )

  • pL HA RCAS (B) ( Plasmid )

  • pLef X ( Plasmid )

  • pLflg RCAS (A) ( Plasmid )

    Flag epitope is a Nhe I - BamHI fragment attached to 3' end of lunatic fringe.

  • pLflg RCAS (B) ( Plasmid )

    Flag epitope is a Nhe I - BamHI fragment attached to 3' end of lunatic fringe.

  • pm-flg-10 ( Plasmid )

  • pMAL C ( Plasmid )

  • pMAL CR 1 ( Plasmid )

  • pMAL P ( Plasmid )

  • pmBMP 7 -FL ( Plasmid )

  • pmCadherin-11 ( Plasmid )

    Internal Sma I; EcoRI; Pst I sites.

  • pmD 8-1 ( Plasmid )

    In situ probe

  • pmFR 1 ( Plasmid )

  • pmFu ( Plasmid )

  • pmHox C4 ( Plasmid )

  • pmHox C5 ( Plasmid )

  • pMiw-cNcad ( Plasmid )

    EcoRI sites flank the insert on each side, but there are also 2 additional EcoRI sites in the vector (one in the RSV enhancer upstream of the insert and one after the SV40 termination signal dowsntream of the insert).

  • pMIW-GFP ( Plasmid )

    This construct has been used successfully by several lab members for electroporation control plasmid and marker. Transient expression of GFP in chick.

  • pMIWIII-GFP ( Plasmid )

    Expresses GFP.

  • pmLmx1 (LMH2) ( Plasmid )

  • pmPLZF ( Plasmid )

  • pmPtc 1 ( Plasmid )

    In situ probe. Amplify by PCR using M13 forward and reverse primers.

  • pmPtc 2 ( Plasmid )

    In situ probe.

  • pMS 3 ( Plasmid )

  • pmSox2 ( Plasmid )

  • pmSu(fu); (IKb) ( Plasmid )

    Internal EcoRI site at ~400bp.

  • pMsx RE2 ( Plasmid )

  • pMsx NT RE 1 ( Plasmid )

  • pMsx RA2 ( Plasmid )

  • pMsx VP16 RA ( Plasmid )

  • pMsx VP16 RE ( Plasmid )

  • pmWnt 3a ( Plasmid )

  • pmWnt 7a RCAS (A) ( Plasmid )

    Replication competent virus.

  • pmWnt 7a RCAS (E) ( Plasmid )

    Replication competent virus

  • pNE 3NB RCAS ( Plasmid )

  • pNFcol2/3'UTR (pMcol2a1-1) ( Plasmid )

  • pNIC myc RCAS (A) ( Plasmid )

  • pNIRES-fu1-800myc-his ( Plasmid )

    Testing NRF-IRES in different cell lines. Fu(1-800) protein is 33kDa. Initiation from NRF-IRES causes doublet proteins in 293T cells.

  • pNIRES-Su(fu) ( Plasmid )

    Su(fu) tagged protein is 57kDa. Translation from NRF-IRES creates doublet protein in 293T cells. Testing NRF IRES in different cell lines.

  • pNL VP16 Slax ( Plasmid )

  • pO6d ( Plasmid )

    Randy Johnson's plasmid library (NG wing + leg, st20-24) original isolate.

  • pO8c ( Plasmid )

    In situ probe template.

  • pOT17 ( Plasmid )

    SEQUENCED - 6/14/01. Sequence from Malcolm named cPotxFL25/3.

  • pOT19 ( Plasmid )

  • pPax9 RCAS (A) ( Plasmid )

  • pPitx1 Slax ( Plasmid )

    Slax shuttle vector construct for cloning to RCAS etc.

  • pPuro (Clonetech) ( Plasmid )

  • pQP 1 ( Plasmid )

  • pR HA RCAS (A) ( Plasmid )

  • pR HA RCAS (B) ( Plasmid )

  • pR RCAS (A) ( Plasmid )

  • pR RCAS (B) ( Plasmid )

  • pRCAS(A) cPitx2Flu ( Plasmid )

    Replication competent cPitx2 virus prep.
    N.B. contains Flu tag at 3' end. Use Nco I for diagnostic digest of insert orientation.

  • pRD 67 (no tag) ( Plasmid )

  • pRflg RCAS (B) ( Plasmid )

    Flag epitope is a Nhe I - BamHI fragment attached to 3' end of radical fringe.

  • pRIAS-CABMPR1A ( Plasmid )

    Replication incompetent retroviral work.

  • pRIAS-CABMPR1B ( Plasmid )

    Replication incompetent retroviral work.

  • pRIASBMP-4 ( Viral plasmid )

    Replication incompetent retrovirus.

  • pRINTS BS Wnt 7 ( Plasmid )

  • prPTHrP receptor (R15B) ( Plasmid )

  • pSam10h ( Plasmid )

  • pShh AP2 ( Plasmid )

  • pSK Cre 1 ( Plasmid )

    Subcloning. In situ probe template. Cre recombinase ORF with 5' SV40 NLS ~1.1 kb.

  • pSK Cre 6 ( Plasmid )

    In situ probe template. Cre recombinase ORF with 5' SV40 NLS ~1.0 kb.

  • pSK-cLtap ( Plasmid )

    In situ probe. Chick ortholog of mouse Ltap. Both genes are homologs of the Drosophila planar polarity gene Strabismus/Van Gogh. Also called chick Vangl2.

  • pSK-Ncad ( Plasmid )

    In situ probe.

  • pSL1180 ( Plasmid )

    Cloning vector. pUC 118 derivative. 3.4kb.

  • pslax mdnTcf4Ben ( Plasmid )

    Shuttle into RCAS.

  • pslax mLef1 ( Plasmid )

    Shuttle into RCAS.

  • pSlax -rep ( Plasmid )

    To make dominant negative constructs using Nco I - EcoRI fragments. EcoRI site: 2 codons [GAA] -glu and [TTC] - phe. Contains engrailed repressor domain, ~960bp.

  • pSlax 13 ( Plasmid )

    Shuttle vector

  • pSlax 13 FLU 2.1 ( Plasmid )

    Slax 13 shuttle vector with FLU and HA tags.

  • pSlax 13 FLAG ( Plasmid )

    Slax 13 shuttle vector with FLAG and HA tag.

  • pSlax 13 VP16 ( Plasmid )

  • pSlax 21 ( Plasmid )

    Shuttle vector.

  • pSlax 30 ( Plasmid )

    Shuttle vector.

  • pSlax cTbx4 ( Plasmid )

    Shuttle vector for RCAS. *Nco I site NOT reconstituted.

  • pSlax Flag His ( Plasmid )

    Shuttle vector with Flag, HA, and His tags.

  • pSlax Flu His ( Plasmid )

    Shuttle vector with flu, His and HA tags.

  • pslax mdnLef1en ( Plasmid )

    Shuttle into RCAS.

  • pSlax mTcf4B ( Plasmid )

    Shuttle into RCAS.

  • pSlax Pitx2 Flu ( Plasmid )

    Shuttle into RCAS.

  • pSlax-Autotaxin ( Plasmid )

    In situ probe.
    Intermediate for cloning into RCAS-vector.

  • pSlax-caronte ( Plasmid )

  • pSlax21-cLtap ( Plasmid )

    Shuttle vector for viral misexpression contructs. The coding sequence of chick Ltap was introduced in two separate cloning steps (5' half, Nco I - BamH I; 3' half, BamH I - Hind III); note that the 3' half of cLtap contains two internal Nco I sites. The full length insert can be removed by digesting with Cla I. Chick Ltap is a homolog of the Drosophila planar polarity gene Strabismus and the ortholog of mouse Ltap, Xenopus Stbm, and zebrafish Stbm. Also called chick Vangl2.

  • pslires mdnLef1en ( Plasmid )

  • pslires mdnTcf4Ben ( Plasmid )

  • pslires mLef1 ( Plasmid )

  • psliresmTcf4B ( Plasmid )

  • pSlug Enr Slax ( Plasmid )

  • pSlug Flu Slax ( Plasmid )

  • pSlug VP16 (4) Slax ( Plasmid )

  • pSNF ( Plasmid )

    Testing NRF-IRES in different cell lines. Su(fu) translation gives 57kDa protein. fu(1-800) translation gives 33kDa protein. Translation from NRF-IRES in 293T cells produces doublet.

  • pSp64 Meis1 ( Plasmid )

    In situ probe (orientation unknown).

  • pTarT-1 ( Gene trap plasmid )

    SA-NIRES-EGFP-pA rBA-Hygro-pA.

  • pTarT-10 ( Gene trap plasmid )

    SA-NIRES-EYFP-pA rBA-DsRed1-pA.

  • pTarT-13 ( Gene trap plasmid )

    SA-NIRES-Koz-Red2-pA rBA-Neo-pA.

  • pTarT-14 ( Gene trap plasmid )

    SA-NIRES-Koz-EGFP-pA rBA-Neo-pA.

  • pTarT-15 ( Gene trap plasmid )

    SA-NRFIRES-DsRED2-pA rBA-Xba1,Mlu1-pA. Self-Inactivating Moloney Murine Sarcoma retroviral backbone.

  • pTarT-16 ( Gene trap plasmid )

    SA-NRFIRES-EGFP-pA rBA-Xba1,Mlu1-pA.

  • pTarT-17 ( Gene trap plasmid )

    SA-NRFIRES-BLAM-pA rBA-Xba1,Mlu1-pA.

  • pTarT-18 ( Gene trap plasmid )

    Splice Acceptor-NRF-IRES-DsRED2-pA rBA-Beta-Lac-pA.

  • pTarT-19 ( Gene trap plasmid )

    SA-NRFIRES-EGFP-pA rBA-Xba1-BLAM-Mlu1-pA.

  • pTarT-2 ( Gene trap plasmid )

    SA-NIRES-EGFP-pA rBA-DsRed.

  • pTarT-20 ( Gene trap plasmid )

    SA-NRFIRES-BLAM-pA rBA-Xba1-Puro-r-Mlu1-pA.

  • pTarT-21 ( Gene trap plasmid )

    SA-NRFIRES-DsRED2-pA rBA-Xba1-EGFP-Mlu1-pA.

  • pTarT-3 ( Gene trap plasmid )

    SA-NIRES-EGFP-pA rBA-DsRed-pA.

  • pTarT-4 ( Gene trap plasmid )

    Gene-trap with polyA trap (Pax2-splice doner) incorporated into the selectable marker. SA-NIRES-EGFP-pA rBA-DsRed-SD. Lab was unable to grow high titer virus with this construct. Probably because the removal of the pA stop for the selectable marker (DsRed) put the 5' LTR in direct competition with the rat Beta-Actin constitutive promoter.

  • pTarT-4-CTL ( Gene trap plasmid )

    Control for pTarT-4 Pas2-Splice Doner is backwards. SA-NIRES-EGFP-pA rBA-DsRed-DS.

  • pTarT-5 ( Gene trap plasmid )

    SA-DsRed2-pA rBA-Neo-pA.

  • pTarT-6 ( Gene trap plasmid )

    SA-EYFP-pA rBA-Neo-pA.

  • pTarT-7 ( Gene trap plasmid )

    SA-NIRES-DsRed2-pA rBA-Neo-pA.

  • pTarT-8 ( Gene trap plasmid )

    SA-NIRES-EYFP-pA rBA-Neo-pA.

  • pTarT-9 ( Gene trap plasmid )

    SA-NIRES-DsRed2-pA rBA-DsRed1-pA.

  • pTbx 5 RCAN ( Plasmid )

    Full-length Tbx 5 in RCAN retroviral vector.

  • pTbx 5 RCAS (A) ( Plasmid )

    Tbx 5 (full-length) retroviral vector.

  • pTbx 5D2Slax ( Plasmid )

    Putative dominant negative Tbx5 in Slax shuttle vector.

  • pTbx15FL (ZAP4) ( Plasmid )

  • pTbx2D2 RCAS (A) ( Plasmid )

    Retroviral vector of c-terminal truncated Tbx2.

  • pTbx2D2 Slax ( Plasmid )

    Putative dominant-negative.

  • pTbx3D2 RCAS(A) ( Plasmid )

    Cla I insert from Tbx3D2 Slax. Retroviral vector for truncated Tbx3 putatve dominant negative.

  • pTbx3D2 Slax ( Plasmid )

    Putative dominant-negative Tbx 3 in slax shuttle vector.

  • pTbx3FLSlax ( Plasmid )

    Slax Shuttle vector of chick Tbx3.

  • pTbx4 RCAN ( Plasmid )

  • pTbx4 RI-Xba ( Plasmid )

    In situ probe template. Derivative of original library isolate; most of 3' UTR removed.

  • pTbx4 SIRES(II) ( Plasmid )

  • pTbx4FluRCAS (A) ( Plasmid )

    Insert from pTbx4 Flu Slax. Flu-tagged (c-terminal) Tbx4 retroviral construct.

  • pTbx4FluSlax ( Plasmid )

    Shuttle vector Flu-tagged (c-terminal) FL clone.

  • pTbx5 D1 RCAS (A) ( Plasmid )

    Putative dominant negative.

  • pTbx5 D1 Slax ( Plasmid )

    Putative dominant negative Tbx5 in Slax shuttle vector. Contains AA of full-length ORF.

  • pTbx5 Enr RCAS (A) ( Plasmid )

    Tbx 5 Enr retroviral vector.

  • pTbx5 Enr Slax ( Plasmid )

    Putative dominant-negative Tbx 5 construct in Slax shuttle vector.

  • pTbx5 FL SIRES II ( Plasmid )

    Full-length Tbx 5 in SIRES II shuttle vector for cloning to RCAN.

  • pTbx5 VpI6(2) RCAS(A) ( Plasmid )

    Retroviral vector of Tbx5 VpI6 (transcriptional activator) fusion.

  • pTbx5 VpI62 Slax ( Plasmid )

    2x copies of l hinge-VpI6 transcriptional activation domain cassette fused to c-terminus of truncated Tbx 5.

  • pTbx5FLSlax ( Plasmid )

    Shuttle vector for cloning to RCAS.

  • PtcFlpe neo/TK ( Plasmid )

    Target flpe to ptc1 - for ES cells.

  • pUAST ( Plasmid )

    Five Gal4 binging sites are inserted, followed by the hsp70 TATA box and transcriptional start, a polylinker, and the SV40 intron and polyadenylation site. Unique sites into which genes, or cDNAs can be inserted include: Eco RI, Blg II, Not I, Xho I, Kpn I, and Xba I. For cloning coding sequences downstream of the Gal UAS.

  • pWnt-1 RCAS A ( Plasmid )

    Contains an internal Cla I site.

  • pWnt-3a RCAS A ( Plasmid )

  • pYwa 13-3 ( Plasmid )

    Orientation of insert can be determined by digestion with Sal I, or Xba I.

  • RCAS (A) Dach2 ( Plasmid )

    pSlax30 + 5' Nco frag Dach2 opened with SalI, EcoRV, removing a 304 bp SalI-EcoRV fragment. Dach2 cut with ScaI, then blunted, then cut with SalI. 1.7 kb 3' fragment isolated. Retroviral misexpression. pSlax30 + 5' Nco frag Dach2 SalI, EcoRV opened vector ligated to 1.7kb SalI-ScaI (blunt)3' Dach2 fragment to create pSlax30- Dach2. 516 bp Dach2 5' NcoI frag cloned into pSlax30 opened with NcoI to create pSlax30 + 5' Nco frag Dach2. pSlax30-Dach2 cut with ClaI and cloned into RCAS (A) Dach2 to create RCAS (A) Dach2.

  • RCAS (A) mSix1 ( Plasmid )

    Retroviral misexpression.

  • RCAS (B) cEya 2 ( Plasmid )

    Retroviral misexpression.

  • RCAS (B) Dach2 ( Plasmid )

    1. 516 bp Dach2 5' NcoI frag cloned into pSlax30 opened with NcoI to create pSlax30 + 5'Nco frag Dach2.

    2. pSlax30 + 5' Nco frag Dach2 opened with SalI, EcoRV, removing a 304 bp SalI-EcoRV fragment.

    3. Dach2 cut with ScaI, then blunted, then cut with SalI. 1.7 kb 3' fragment isolated.

    4. pSlax30 + 5'Nco frag Dach2 SalI, EcoRV opened vector ligated to 1.7kb SalI-ScaI (blunt)3' Dach2 fragment to create pSlax30- Dach2.

    5. pSlax30-Dach2 cut with ClaI and cloned into RCAS (B) Dach2 to create RCAS (B) Dach2. Retroviral misexpression.

  • RCAS - cWnt 11 ( Plasmid )

  • RCAS - DANr ( Plasmid )

  • RCAS - ires Car ( Plasmid )

  • RCAS - ires II - Nodal ( Plasmid )

  • RCAS - mshh - CD4 ( Plasmid )

  • RCAS AP (A) ( Plasmid )

    RCAS A coat with alkaline phosphatase.

  • RCAS AP (E) ( Plasmid )

    RCAS E coat with alkaline phosphatase.

  • RCAS AP(B) ( Plasmid )

    Used for avian virus production; RCAS B coat with alkaline phosphatase.

  • RCAS BP (B) ( Plasmid )

    Avian viral production.

  • RCAS BP (E) ( Plasmid )

    Avian virus production.

  • RCAS BP(A) ( Plasmid )

    Avian virus production.

  • RCAS enrepnkx2.3 ( Retroviral plasmid )

    Replication competent retroviral vector.

  • RCAS enrepNkx2.5 ( Retroviral plasmid )

    Replication competent retrovirus.

  • RCAS(A) Gremlin ( Retroviral plasmid )

  • RCAS(A) mMC1-R ( Plasmid )

    Missexpression resulted in cartilage defects that were most pronounced in the ribs. The initially normal cartilage accumulated curves, complete turns and breaks. A similar phenotype was seen with a virus expressing the E92k activating mutation of the receptor.

  • RCAS(A)-cLtap ( Plasmid )

    Replication competent viral misexpression.

  • Rcas(A)-ScxEnR ( Plasmid )

    Scleraxis was subcloned as an NcoI, RI fragment into the En-Slax13 vector. The Engrailed repressor is at the C terminus.

  • Rcas(A)-ScxEnRCD ( Plasmid )

    The En-Slax13 vector includes domains A-D of the Engrailed repressor (1000bp). Some papers suggest that the CD domains alone may be more potent. The Engraikled repressor in SlaxScx-En was replaced by a fragment that includes only the CD domains.

  • Rcas(A)-ScxHLHEnR ( Plasmid )

    The bHLH of Scleraxis was PCRed by fusing an ATG to bp175-390 of the ORF of chick scleraxis. This fragment was subcloned as an NcoI, RI fragment into the En-Slax13 vector. The Engrailed repressor is at the C terminus.

  • Rcas(A)-ScxHLHEnRCD ( Plasmid )

    Same as ScxEnRCD (stock no. 748), but with the bHLH of Sxcleraxis only.

  • Rcas(A)-ScxVP16 ( Plasmid )

    Scleraxis was cloned as an NCOI, RI fragment into the Slax-VP16 vector.

  • Rcas(A)-ScxWRPW ( Plasmid )

    The transcriptional corepressor Groucho attaches in some cases to a WRPW motif at the C terminus of a protein. Tailing a TF with WRPW can therefore convert it into a repressor. Infection with the virus led to the production of ectopic AER's. Probably a neomorph that is not related to the function of scleraxis. A possible dominant negative.

  • RCAS(B)-cLtap ( Plasmid )

    replication competent viral misexpression

  • RCAS-mLef ( Plasmid )

  • RCASA mdnTcf4Ben ( Plasmid )

  • RCASA mdnLef1en ( Plasmid )

  • RCASA mTcf4B ( Plasmid )

  • RCASB mdnLef1en ( Plasmid )

  • RCASB mdnTcf4Ben ( Plasmid )

  • RCASB mTcf4B ( Plasmid )

  • RIA ATX ( Plasmid )

    Autotaxin was too big for RCAS and the replication competent virus underwent recombination events. To attempt missexpression ATX was cloned in RIA using the Slires-11 as a shutle vector. The cloning into RIA was very difficult (rearrangements in the plasmid).

  • RIA-ires II hSmo M1 ( Plasmid )

  • RIA-ires II hSmo M2 ( Plasmid )

  • RIA-ires II hSmo WT ( Plasmid )

  • RIS mdnTcf4Ben ( Plasmid )

  • RISAP act[beta]cat ( Plasmid )

  • RISAP mdnLef1en ( Plasmid )

  • RISAP mdnTcf4Ben ( Plasmid )

  • rPTHrP-R pBS ( Plasmid )

    In situ probe.

  • SA-IRES-AP-SV40 ( Plasmid )

    Expression cassette for AP transgenic.

  • Scx-GFP MEFS ( Primary fibroblast cell line )

    Mouse fibroblast line expressing Scx-GFP used for tenocyte differentiation screening.

  • ScxCTV ( Plasmid )

  • ScxgCre ( Plasmid )

    Scleraxis Cre transgenic

  • ScxgEgfp ( Plasmid )

    A Scleraxis GFP transgenic vector. Can be isolated as a single fragment using NotI or SalI.

  • Scxgen12 ( Plasmid )

  • Shh cre ERT2 ( Plasmid )

    Target cre ERT2 to Shh -for ES cells.

  • Shh exon 2 ( Plasmid )

    In situ probe for floxed conditional Shh allele - senses the floxed part of the gene.

  • Shh gfp cre ( Plasmid )

    Target GFP Cre to Shh - for ES cells.

  • Shh KO vector ( Plasmid )

    original Shh null targeting construct

  • Slax GW48 ( Plasmid )

    Virus resulted in no observable phenotype.

  • Slax NHAB100 ( Plasmid )

    XcmI cuts in Slax upstream of the NcoI site. Missexpressoin of the NHAB100 virus did not result in observable phenotypes.

  • Slax NHAB163 ( Plasmid )

  • Slax NHAB4 ( Plasmid )

    Viral infection did not cause an observable phenotype.

  • spkB1 ( Plasmid )

    In situ probe for Pbx1a (orientation unknown).

  • SV 40 splice acceptor - poly A ( Plasmid )

    Used in making UAS transgenic construct.

  • Sx-ires II-XCerberus S ( Plasmid )

  • T Backbone ( Plasmid )

    Transgenic backbone.

  • TREX-226-NIRES ( Gene trap plasmid )

  • TREX-Hyg-NIRES ( Gene trap plasmid )

  • TREX-Hygro ( Gene trap plasmid )

  • TREX-NIRES-Koz ( Gene trap plasmid )

  • VP162 Slax ( Plasmid )

    VP16 domains are VP16 modified - Accession # U55941.1. For construction of VP16 Fusion constructs. Clone into Nco I - RI. VP16 - l hinge fusions - two copies.

  • VP164 Slax ( Plasmid )

    VP16 domains are VP16 modified - Accession # U55941.1. For construction of VP16 Fusion constructs. Clone into Nco I - RI. VP16 - l hinge fusions - four copies.

  • Wnt8c-RCAS(A) ( Plasmid )

    Retroviral misexpression.

  • xDsh-PDZ-RCAS(A) ( Plasmid )

    Retroviral misexpression. Functional.

  • xDshDEP+ RCAS(A) ( Plasmid )

    Retroviral misexpression. Functional.

  • ß-cat(4)pGEMT ( Plasmid )

    In-situ probe.


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Last updated: 2013-05-15T15:34:44.566-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016