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DRSC Collection for Follow-Up with Distinct Reagents (DRSC Follow-Up)

eagle-i ID

http://harvard.eagle-i.net/i/00000152-13af-39d6-6771-1ce080000000

Resource Type

  1. RNAi library

Properties

  1. Resource Description
    DRSC Follow-Up is a collection of amplicons for RNAi knockdown of genes that have previously scored as hits in any screen at the DRSC. As of spring 2008, DRSC Follow-Up covered about 6,000 amplicons. Amplicons directed against unrepresented genes are generated when new lists of screen hits submitted by screeners. Our ultimate goal is for genome-wide coverage. Importantly, the DRSC Follow-Up amplicons are distinct from the amplicons in the 2.0 amplicons (i.e. target a different region of the gene), providing independent validation of a positive RNAi result. Please note that confirmation of the RNAi result does not eliminate the need for other follow-up, such as in vivo confirmation of biological relevance.
  2. Access Restriction(s)
    Screen hits can be requested as "cherry picks" from the DRSC Follow-Up collection. The requested amplicons are provided as PCR-amplified DNA templates. These can be used for dsRNA production at the home institution.
  3. Website(s)
    http://www.flyrnai.org/DRSC-DRS.html
  4. Library Count
    6,000 amplicons
  5. Location
    Drosophila RNAi Screening Center (HMS)
 
RDFRDF
 
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016