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DRSC Collection for Follow-Up with Distinct Reagents (DRSC Follow-Up)

eagle-i ID


Resource Type

  1. RNAi library


  1. Resource Description
    DRSC Follow-Up is a collection of amplicons for RNAi knockdown of genes that have previously scored as hits in any screen at the DRSC. As of spring 2008, DRSC Follow-Up covered about 6,000 amplicons. Amplicons directed against unrepresented genes are generated when new lists of screen hits submitted by screeners. Our ultimate goal is for genome-wide coverage. Importantly, the DRSC Follow-Up amplicons are distinct from the amplicons in the 2.0 amplicons (i.e. target a different region of the gene), providing independent validation of a positive RNAi result. Please note that confirmation of the RNAi result does not eliminate the need for other follow-up, such as in vivo confirmation of biological relevance.
  2. Access Restriction(s)
    Screen hits can be requested as "cherry picks" from the DRSC Follow-Up collection. The requested amplicons are provided as PCR-amplified DNA templates. These can be used for dsRNA production at the home institution.
  3. Website(s)
  4. Library Count
    6,000 amplicons
  5. Location
    Drosophila RNAi Screening Center (HMS)
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016