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GHY285 (hisG replacement of ppr2)

eagle-i ID

http://harvard.eagle-i.net/i/0000012d-c2d3-dc77-4882-b08d80000000

Resource Type

  1. Saccharomyces cerevisiae

Properties

  1. Resource Description
    GHY285 is the TFIIS-minus strain from Grant Hartzog . This strain was obtained by hisG replacement of ppr2 gene. This replacement was accomplished by the transforming the basic Fred Winston strain FY653 (mat alpha his4-912-delta lys2-128-delta leu2-delta-1 ura3-52) with the ppr2-hisG plasmid pJD3 constructed in Caroline Kane's lab (UC-Berkley).In order to cross this strain with Z280, I looped out the hisG-Ura3 by growing GHY285 at the FOA plate and used the revertants for the cross.
  2. Resource Description
    Mat alpha his4-912-delta lys2-128-delta leu2-delta-1 ura3-52ppr2::hisG-Ura3.
  3. Related Publication or Documentation
    TFIIS enhances transcriptional elongation through an artificial arrest site in vivo.
  4. Parental Strain Name
    GHY285
  5. Location
    Struhl Laboratory
 
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Provenance Metadata About This Resource Record

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