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DNA/miRNA quality analysis

eagle-i ID

http://harvard.eagle-i.net/i/0000012d-80c3-5e9b-4882-b08d80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "A major problem in the use of banked tissue is the variability of recovery and quality of RNA. Experienced users of the various local brain banks report an approximate yield of less than 50% of the material they need. While the underlying biologic issues that explain this poor yield are complicated, it is extremely important to assess nucleic acid quality of a specimen before investing significant time and other resources in subsequent analysis. To provide this 'quality control', the ATRC has an Agilent 2100 Bioanalyzer for unbiased assessment of RNA/DNA quality of tissue samples. miRNA-sized small RNAs can also be visualized with the Agilent bioanalyzer. We have studied the relationship between total RNA stability and miRNA stability using a combination of Agilent bioanalysis, rtPCR and the FlexMiR assay. As such, we are able to reliably assess the quality of miRNA samples prior to expression analysis."
  3. Service Fee URL
    http://www.neurodiscovery.harvard.edu/images/stories/atrc_user_fees.pdf
  4. Contact
    Vanderburg, Charles, Ph.D.
  5. Service Provided by
    Harvard NeuroDiscovery Center - Advanced Tissue Resource Center (HMS)
  6. Website(s)
    http://www.neurodiscovery.harvard.edu/resources/atrc.html
  7. Related Technique
    RNA quality analysis
  8. Related Technique
    DNA quality analysis
 
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016