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HA-Rpb3 integrating plasmid (URA)

eagle-i ID

http://harvard.eagle-i.net/i/0000012d-751c-c7a4-87dd-920c80000000

Resource Type

  1. Plasmid

Properties

  1. Construct Insert
    RPB3
  2. Resource Description
    ZM313 (EcoRV/HindIII) + 5'-most 400 bp POB3 ORF (PCR, EcoRV/HindIII) = ZM313-POB32. ZM303-POB3 (EcoR/BsaA1) + 700 bp fragment encompassing RPB3 promoter-HA-5' ORF (from Z280) (EcoRI/EcII36II).
  3. Construct Backbone
    URA3
  4. Related Publication or Documentation
    The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo.
  5. Related Technique
    Chromatin immunoprecipitation
  6. Related Technique
    Transcription profiling
  7. Related Technique
    Immunoprecipitation
  8. Location
    Struhl Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016