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YIplac204 CUP1-YLR454
eagle-i ID
http://harvard.eagle-i.net/i/0000012d-751c-881e-87dd-920c80000000
Resource Type
Properties
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Construct Insert
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Hpr1
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Construct Insert
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Tho2
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Resource Description
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Linearize via KpnI500bp CUP1 PvuII/BstYI promoter fragment from pYEX 4T into YIp204 GAL1-YLR454 (p8-2) via Ec136II/BamHI (~700 bp GAL10/1 fragment thus removed).
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Construct Backbone
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YIplac204
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Related Publication or Documentation
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The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo.
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Selectable Marker
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TRP1
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Related Technique
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In vitro transcription
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Related Technique
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Chromatin immunoprecipitation
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Location
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Struhl Laboratory
Harvard University
