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YIplac204 GRE2-YLR454
eagle-i ID
http://harvard.eagle-i.net/i/0000012d-751c-6a5f-87dd-920c80000000
Resource Type
Properties
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Construct Insert
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Hpr1
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Construct Insert
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Tho2
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Resource Description
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Linearize via KpnI900bp GRE2 promoter pcr product (blunt end) into YIp204 GAL1-YLR454 (p8-2) via EcoRI/BamHI/Klenow (~700 bp GAL10/1 fragment thus removed).
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Construct Backbone
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YIplac204
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Related Publication or Documentation
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The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo.
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Selectable Marker
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TRP1
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Related Technique
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Northern blot analysis
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Related Technique
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Run-on transcription assay
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Related Technique
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Immunoprecipitation
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Location
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Struhl Laboratory
