YIplac204 GRE2-YLR454

eagle-i ID

Resource Type

Properties

1. Construct Insert

   Hpr1

2. Construct Insert

   Tho2

3. Resource Description

   Linearize via KpnI900bp GRE2 promoter pcr product (blunt end) into YIp204 GAL1-YLR454 (p8-2) via EcoRI/BamHI/Klenow (~700 bp GAL10/1 fragment thus removed).

4. Construct Backbone

   YIplac204

5. Related Publication or Documentation

   The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo.

6. Selectable Marker

   TRP1

7. Related Technique

   Northern blot analysis

8. Related Technique

   Run-on transcription assay

9. Related Technique

   Immunoprecipitation

10. Location

    Struhl Laboratory