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YIplac204 GRE2-YLR454

eagle-i ID

http://harvard.eagle-i.net/i/0000012d-751c-6a5f-87dd-920c80000000

Resource Type

  1. Plasmid

Properties

  1. Construct Insert
    Hpr1
  2. Construct Insert
    Tho2
  3. Resource Description
    Linearize via KpnI900bp GRE2 promoter pcr product (blunt end) into YIp204 GAL1-YLR454 (p8-2) via EcoRI/BamHI/Klenow (~700 bp GAL10/1 fragment thus removed).
  4. Construct Backbone
    YIplac204
  5. Related Publication or Documentation
    The FACT complex travels with elongating RNA polymerase II and is important for the fidelity of transcriptional initiation in vivo.
  6. Selectable Marker
    TRP1
  7. Related Technique
    Northern blot analysis
  8. Related Technique
    Run-on transcription assay
  9. Related Technique
    Immunoprecipitation
  10. Location
    Struhl Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record

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