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pJD4 = pUC-ume6::hisG

eagle-i ID

http://harvard.eagle-i.net/i/0000012d-7512-3a3f-87dd-920c80000000

Resource Type

  1. Plasmid

Properties

  1. Construct Insert
    Ume6::hisG
  2. Resource Description
    replaces AA 1-594 of Ume6 with hisG-URA3digest with EcoRI, MluI to integrate
  3. Construct Backbone
    pUC
  4. Related Publication or Documentation
    Histone acetylation at promoters is differentially affected by specific activators and repressors.
  5. Selectable Marker
    all hisG replacements are selected for on -URA, then URA 3 marker is looped out
  6. Related Technique
    Transcriptional activation assay
  7. Related Technique
    Chromatin immunoprecipitation
  8. Location
    Struhl Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    etl
  3. created
    2011-01-11T06:34:46.923-06:00
  4. creator
    rgarces (Robert Garces)
  5. mediator
    etl
  6. modified
    2011-03-31T16:21:22.485-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016