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pJD1 = pBS-hisG

eagle-i ID

http://harvard.eagle-i.net/i/0000012d-7511-f2fb-87dd-920c80000000

Resource Type

  1. Plasmid

Properties

  1. Construct Insert
    His3
  2. Resource Description
    All hisG replacements are selected for on -URA, then URA 3 marker is looped out.
  3. Resource Description
    HisG BamHI-BglII fragment into BamHI of pBSbased on pNK51.
  4. Construct Backbone
    pBS
  5. Related Publication or Documentation
    Histone acetylation at promoters is differentially affected by specific activators and repressors.
  6. Related Publication or Documentation
    A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.
  7. Related Technique
    Transcriptional activation assay
  8. Related Technique
    Chromatin immunoprecipitation
  9. Location
    Struhl Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    etl
  3. created
    2011-01-11T06:34:33.008-06:00
  4. creator
    rgarces (Robert Garces)
  5. mediator
    etl
  6. modified
    2011-03-31T16:21:08.181-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016