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pL27 = YIplac211 3HA TAF150 (aka TAF2)

eagle-i ID

http://harvard.eagle-i.net/i/0000012d-7509-4534-87dd-920c80000000

Resource Type

  1. Plasmid

Properties

  1. Construct Insert
    3HA-TAF150
  2. Resource Description
    Constructed by cloning a EcoRI-PstI fragment comprised of nucleotides +2860 to +5179 of TAF150 with HA epitopes inserted just before the stop codon. For targeting integration at TAF150 loci, pL27 was digested by HindIII.
  3. Construct Backbone
    YIplac211
  4. Related Publication or Documentation
    Binding of TBP to promoters in vivo is stimulated by activators and requires Pol II holoenzyme.
  5. Selectable Marker
    URA3
  6. Related Technique
    Transcriptional activation assay
  7. Related Technique
    Chromatin immunoprecipitation
  8. Related Technique
    Coimmunoprecipitation
  9. Related Technique
    Western blot analysis
  10. Location
    Struhl Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    etl
  3. created
    2011-01-11T06:24:59.890-06:00
  4. creator
    rgarces (Robert Garces)
  5. mediator
    etl
  6. modified
    2011-03-31T16:14:20.970-05:00

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