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Human cell line identity verification

eagle-i ID

http://harvard.eagle-i.net/i/0000012c-ccad-7c68-a061-4a6580000000

Resource Type

  1. Material analysis service

Properties

  1. Resource Description
    "Cultured cells used in research can become altered in culture, either by contamination from other cell lines or by spontaneous genetic changes after a high number of subcultures. The American Type Culture Collection (ATCC) recommends using low passage cell lines and authentication of cell lines used in research to assure reliable and reproducible results. The Molecular Diagnostics Laboratory has adopted the Promega PowerPlex 1.2 system to authenticate human cell lines as a service to investigators. The PowerPlex 1.2 system uses a multiplex PCR approach to coamplify 8 highly polymorphic STRs and the Amelogenin gene in the human genome. The amplified DNA fragments of each sample are resolved on the Applied Biosystems 3100 Genetic Analyzer with Applied Biosystems Genotyper 2.0 software and the Promega PowerTyper TM 1.2 Macro file to determine cell line genotypes. Please note that this genotyping method assays 9 (for female) or 10 (for male) locations in the genome. Changes that may have arisen at other loci during cell culture will not be detected by this method."
  2. Service Provided by
    Shannon McCormack Advanced Molecular Diagnostics Laboratory (DFCI)
  3. Website(s)
    http://mdl.dfci.harvard.edu/http/public/cell_line_identity_verification.html
 
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016