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miRNA expression profiling

eagle-i ID


Resource Type

  1. Material analysis service


  1. Resource Description
    "Total RNA or low molecular weight-enriched RNA is converted into cDNA using a single 'megaplex' reverse transcriptase reaction. The megaplex reaction contains a specific stem-loop primer for each of the mature target microRNA's (Each stem-loop primer is designed to hybridize to only the fully mature microRNA, and not to precursor forms of its target.). The cDNA is subjected to a 'pre-amplification' PCR. The diluted pre-amplification products serve as substrates for TaqMan quantitative PCR reactions. The assays that comprise this process are manufactured by Applied Biosystems."
  2. Service Provided by
    Shannon McCormack Advanced Molecular Diagnostics Laboratory (DFCI)
  3. Website(s)
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016