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RNA purification service

eagle-i ID

http://harvard.eagle-i.net/i/0000012c-bced-4a4d-02c0-2b6880000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "RNA purification in the MGCF is performed using the PAXgene Blood RNA system. The PAXgene tubes contain an RNA stabilizer that allows the blood to be stored without RNA degradation. Customers provide the Core with their collected PAXgene blood RNA Tube and the Core performs the extractions on the QIAcube instrument using the PAXgene Blood RNA Kit, which utilizes a silica-membrane-based spin-column technology. Whole genome amplification is performed using Qiagen REPLI-g Kits. REPLI-g relies on multiple displacement amplification technology and phi29 DNA polymerase to create highly uniform DNA amplification across the entire genome with minimal sequence bias."
  3. Service Fee URL
    http://core.iddrc.org/molecular-genetics/?page_id=23#Pricing
  4. Related Resource
    Qiagen QIAcube
  5. Service Provided by
    Molecular Genetics Core Facility (BCH)
  6. Website(s)
    http://core.iddrc.org/molecular-genetics/
  7. Related Technique
    Purification
 
RDFRDF
 
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016