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pBS MCK CRE

eagle-i ID

http://harvard.eagle-i.net/i/0000012a-2518-2d98-5617-79428000003b

Resource Type

  1. Plasmid

Properties

  1. Resource Description
    Contains Cre Recombinase.
  2. Resource Description
    Cre is driven by the Muscle Creatine Kinase promoter; To create a construct for muscle-specific expression of the Cre recombinase, a 6.5 kb XhoI fragment containing 3 kb of the MCK promoter and enhancer 1, untranslated exon 1, 3 kb of intron 1 including the enhancer region E2, and the first 16 bp of exon 2 was subcloned into the XhoI site of pBluescriptKS to result in pBSMCK (see Addgene plasmid #12528 for details. Plasmid 12528 contains a polyA sequence, whereas the base vector that MCK cre was built from does not - the polyA was added using a different cloning strategy as described below.). A 1.3 kb blunt-ended SacI/SacII fragment containing a modified Cre cDNA with a SV-40 large T antigen nuclear localization signal inserted in-frame after the ATG of Cre and a poly(A) signal was inserted into the blunt-ended PstI site of pBSMCK to result in pMCKNlsCrepolyA.
  3. Resource Description
    Transgenic construct.
  4. Construct Backbone
    pBluescript KS
  5. Related Publication or Documentation
    A muscle-specific insulin receptor knockout exhibits features of the metabolic syndrome of NIDDM without altering glucose tolerance.
  6. Selectable Marker
    Ampicillin
  7. Location
    Ron Kahn Laboratory
 
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