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pTarT-4

eagle-i ID

http://harvard.eagle-i.net/i/0000012a-250b-df2e-5617-7942800001d2

Resource Type

  1. Gene trap plasmid

Properties

  1. Resource Description
    Gene-trap with polyA trap (Pax2-splice doner) incorporated into the selectable marker. SA-NIRES-EGFP-pA rBA-DsRed-SD.
  2. Resource Description
    Lab was unable to grow high titer virus with this construct. Probably because the removal of the pA stop for the selectable marker (DsRed) put the 5' LTR in direct competition with the rat Beta-Actin constitutive promoter.
  3. Additional Name
    Gene Trap
  4. Construct Backbone
    pTarT
  5. Selectable Marker
    Chloramphenicol
  6. Related Technique
    Gene trapping
  7. Location
    Tabin Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016